饲粮添加低聚木糖对肥育猪血浆生化参数和肉品质的影响

Effects of Dietary Supplementation with Xylo-oligosaccharide on Plasma Biochemical Parameters and Meat Quality in Finishing Pigs

为研究低聚木糖对肥育猪血浆生化参数和肉品质的影响,本试验选取平均体重为65 kg的三元杂交猪50头,随机分为5组,每组10头,公、母各半,单栏饲养。试验设置对照组(饲喂基础饲粮)、抗生素组(在每吨基础饲粮中添加0.2 kg抗敌素和0.04 kg有效含量为50%的维吉尼亚霉素预混剂)、低聚木糖组(在基础饲粮中分别添加100、250、500 g/t低聚木糖)。于试猪平均体重达100 kg时,前腔静脉采血,分离血浆,测定生化参数;屠宰后取背最长肌,测定肉品质、肌纤维类型和肌肉生长相关基因表达量。结果表明:与对照组或抗生素组相比,添加100 g/t低聚木糖可显著增加碱性磷酸酶活性(P<0.05),添加100、250 g/t低聚木糖可显著降低白蛋白/球蛋白(P<0.05);添加低聚木糖可显著提高背最长肌粗蛋白质含量,添加100 g/t或500 g/t低聚木糖可显著增加背最长肌中赖氨酸、苏氨酸、亮氨酸、异亮氨酸、丝氨酸、丙氨酸、必需氨基酸、鲜味氨基酸和总氨基酸含量(P<0.05),添加100 g/t或250 g/t低聚木糖可显著上调背最长肌中生肌决定因子和肌肉生长抑制素表达量(P<0.05)。综上所述,饲粮添加低聚木糖可调控机体氮代谢相关生化参数、增加肌肉粗蛋白质和氨基酸含量、上调生肌决定因子和肌肉生长抑制素表达量,进而改善肉品质和营养价值,且添加100 g/t时效果较佳。

The present study was conducted to investigate the effects of dietary supplementation with xylo-oligosaccharide(XOS)on plasma biochemical parameters and meat quality in finishing pigs.A total of 50 Duroc×Large White×Landrace pigs with about 65 kg of initial body weight(BW)were randomly assigned to one of five groups with 10 pigs,and fed individually.The experimental groups included control group,antibiotic group,supplementation groups of 100,250 or 500 g/t XOS.When the average BW of pigs reached about 100 kg,blood samples were collected by precaval vein,and then the plasma were obtained for analyzing biochemical parameters.The samples of longissimus dorsi(LD)muscle were collected,and meat quality,muscle chemical composition,mRNA expression levels of muscle fiber types and muscle growth-related genes were measured.The results showed that,when compared with the control or antibiotic group,dietary supplementation with 100 g/t XOS increased alkalinephosphatase activity(P<0.05),and supplementation with 100 or 250 g/t XOS decreased albumin/globulin(P<0.05).Dietary supplementation with XOS increased(P<0.05)the crude protein content of LD muscle(P<0.05);supplementation with 100 or 500 g/t XOS increased(P<0.05)the muscular contents of Lys,Thr,Leu,Ile,Ser,Ala,essential amino acid,fresh amino acid,and total amino acid;supplementation with 100 or 250 g/t XOS up-regulated(P<0.05)the mRNA expression of Myogenic Determination(MyOD)and Myostatin(MSTN).Collectively,these findings suggest that dietary supplementation with different doses of XOS can regulate nitrogen metabolism-related biochemical parameters,and improve the pork nutritional value via increasing the muscular contents of crude protein and amino acids,as well as upregulating the expression levels of MyOD and MSTN genes,which improve the meat quality and nutritive value of pork;the efficiency of dietary supplementation with 100 g/t XOS is optimal in finishing pigs.