岩白菜素对肝癌的抑制作用

Inhibitory role of bergenin on hepatic cancer

目的评价岩白菜素对肝癌HepG2细胞和大鼠肝癌模型的抑制作用。方法使用一定浓度梯度的岩白菜素(0、0.1、0.2、0.4、0.6、0.8mmol/L)与肝癌HepG2细胞共孵育,评价岩白菜素对HepG2细胞增殖、平板克隆、迁移和侵袭能力的影响,同时利用二乙基亚硝胺(DEN)连续喂饲的方法构建大鼠肝癌模型,在离建模成功的2周开始,实验组予以10mg/kg岩白菜素连续灌胃2周,对照组只喂饲等量岩白菜素溶剂二甲基亚砜(DMSO),观察岩白菜素对大鼠肝癌大小、大鼠存活时间的影响。结果岩白菜素能显著抑制肝癌HepG2细胞的增殖(P=0.029 9)、克隆形成(P<0.01)、迁移和侵袭能力(P=0.009 8和P=0.006 9),并呈剂量依赖性。岩白菜素亦能抑制大鼠肝癌的生长,使肝癌体积减少(P<0.01),大鼠存活时间延长(P<0.05)。结论岩白菜素对肝癌有抑制作用。

Objective To evaluate the inhibitory role of bergenin on hepatic cancer HepG2 cells and rat liver cancer model.Methods A certain concentration gradient of bergenin(0,0.1,0.2,0.4,0.6,0.8 mmol/L)was used to incubate with human liver cancer cell line HepG2.The effects of bergenin on the proliferation of HepG2 cells,plate clone,migration and invasion ability were evaluated.Meanwhile the diethylnitrosamine(DEN)continuous feeding method was utilized to construct liver cancer rat model.Rat liver cancer model was used to test the inhibitory role of bergenin on liver cancer.At 2 weeks from the beginning of model construction success,the experimental group was given 10mg/kg berginin by continuous gavage for 2 weeks,while the control group was fed only by equal quantity of berginin solvent dimethyl sulfoxide(DMSO).Then the effects of berginin on liver cancer size and survival time were observed.Results Bergenin could significantly suppress the proliferation of liver cancer cells(P=0.029 9),colony forming(P<0.01),migration and invasion ability(P=0.009 8 and P=0.006 9),moreover which showed the dose dependent manner.Bergenin also could inhibit the growth of rat liver cancer,made its volume to be decreased(P<0.01)and rat survival time to be prolonged(P<0.05).Conclusion Bergenin has the inhibitory effect on liver cancer.