300例基质金属蛋白酶-91562基因多态性与急性脑梗死的关系探讨

Study on relationship between matrix metalloproteinase-91562 gene polymorphism and acute cerebral infarction in 300 cases

目的探讨基质金属蛋白酶-9(MMP-9)启动子区C-1562T的基因多态性与急性脑梗死的关系。方法运用多聚酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)技术检测300例急性脑梗死患者(病例组)和300例同期健康体检者(对照组)MMP-9启动子区的1562C/T的基因多态性,并进行基因测序,检测MMP-9C-1562T的基因型,并将病例组分为大动脉粥样硬化型(LAA)、小动脉闭塞型(SAO)和其他原因型(SOE)。结果 MMP-9启动子区的1562C/T各种基因型频率及等位基因频率在病例组和对照组之间差异无统计学意义(P>0.05)。进一步对脑梗死进行亚型分组发现,MMP-9启动子区的1562C/T各种基因型频率及等位基因频率在脑梗死亚型间差异亦无统计学意义(P>0.05)。多因素Logistic回归分析结果显示,在调整传统危险因素后,MMP-9启动子区的1562C/T基因多态性并不增加脑梗死的发病风险。结论 MMP-9启动子C-1562T的基因多态性与急性脑梗死无明显相关性。

Objective To explore the relationship between promoter region 1562C/T polymorphisms of matrix metalloproteinase-9(MMP-9)and acute cerebral infarction in Henan Han population.Methods The MMP-9 promoter region 1562C/T polymorphisms was detected in 300 cases of acute cerebral infarction(case group)and contemporaneous 300 persons undergoing healthy physical examination(control group)by using PCR-RELP.The gene sequencing was performed.The genotypes of MMP-9 C-1562T were detected.The case group was re-divided into the large-artery atherosclerosis(LAA)type,small-artery occlusion(SAO)type and stroke of other etiology(SOE).Results The frequencies of various genotypes and alleles in MMP-9 promoter region 1562C/T showed no statistically significant difference between the control group and case group(P>0.05).The further subtype analysis on cerebral infarction found that the frequencies of various genotypes and alleles in MMP-9 promoter region 1562C/T had no statistically significant difference among different cerebral infarction subtypes(P>0.05).The multivariate Logistic regression results showed that after adjusting the conventional risk factors,the polymorphism of MMP-9 promoter region 1562C/T gene did not increase the onset risk of cerebral infarction.Conclusion The MMP-9 promoter C-1562T gene polymorphism has no obvious correlation with acute cerebral infarction.