树鼩胆石模型与消化道P物质相关的研究

Substance P in the digestive tract of a tree shrew cholesterol-induced gallstone model

目的探讨高胆固醇饲料饲喂树鼩建立树鼩胆石模型与P物质(substance P,SP)的关系。方法通过建立树鼩胆石模型,检测消化道SP免疫组化的表达,模型组与对照组进行比较分析。结果模型组与对照相比,树鼩消化道SP免疫组化表达含量比对照组低(P<0.05),树鼩胆石模型与SP表达水平呈负相关。结论树鼩胆囊结石与消化道分泌激素调节机制有关。

Objective To investigate the possibility of establishing the high-cholesterol forage-feeding tree shrew as a cholesterol-induced gallstone model and to determine the association of substance P(SP)with this model.Methods Upon establishment of the tree shrew cholesterol-induced gallstone model,the SP expression of the digestive tract was detected with immunohistochemistry method and compared among the model groups and the control group.Results In the model groups,immunohistochemistry showed that gastrointestinal SP expression was lower than that in the control group(P<0.05).The tree shrew gallstone model was negatively correlated with SP expression level.Conclusions Tree shrew gallstones are associated with the regulation mechanisms of hormone secretion in the digestive tract.