周龄对非肥胖型糖尿病背景小鼠体外受精效果的影响

Effects of age on efficacy of in vitro fertilization in non-obese diabetic mice

目的提高非肥胖型糖尿病(non-obese diabetic,NOD)背景小鼠体外受精(in vitro fertilization,IVF)效果。方法使用不同周龄的NOD背景雌鼠进行实验,实验分为四组,分别为4、6、8和10周龄雌鼠,与3~6月龄NOD雄鼠进行体外受精,第二天统计每只雌鼠可用胚胎数量,重复两次实验。收集2016—2018年NOD小鼠移植生仔数据进行比较。结果第一次实验4、6、8和10周龄四组每只雌鼠体外受精后可用二细胞胚胎数以平均数±标准差表示,分别为(13.4±2.5)枚、(22.7±2.9)枚、(22.0±7.0)枚、(30.5±10.9)枚。第二次实验4、6、8和10周龄四组每只雌鼠可用二细胞胚胎数以平均数±标准差表示,分别为(7.8±2.6)枚、(16.5±4.5)枚、(14.2±6.5)枚、(24.5±5.9)枚。4、8、10周龄雌鼠体外受精后胚胎移植平均生仔率±标准差分别为(35.32±9.9)%、(36.19±6.2)%、(43.15±6.9)%。结论 10周龄的NOD背景雌鼠进行体外受精效果最好,建议使用10周龄雌鼠进行体外受精。

Objective To improve the results of in vitro fertilization(IVF)for non-obese diabetic(NOD)mice.Methods NOD female mice were divided into four age groups:4,6,8,and 10 weeks old.Sperm from 3-6-month-old male NOD mice were used for in vitro fertilization.The next day,the number of usable embryos per female mouse was counted and the experiment was repeated twice.NOD mouse embryo transfer data collected from 2016-2018 were used for the comparison of pup rates.Results Twenty-four hours after the first experiment,the means±SD of the number of two-cell-stage embryos per group were 13.4±2.5,22.7±2.9,22±7.0,and 30.5±10.9,respectively.Twenty-four hours after the second experiment,the numbers of two-cell-stage embryos per group were 7.8±2.6,16.5±4.5,14.2±6.5,and 24.5±5.9,respectively.The mean±SD rates of pups born from transferred embryos after in vitro fertilization in 4,8,and 10 weeks were(35.32±9.9)%,(36.19±6.2)%,and(43.15±6.9)%,respectively.Conclusions Ten-week-old NOD mice respond better to IVF than 4-,6-,or 8-week-old ones.Therefore,we suggest the selection of 10-week-old NOD female mice for in vitro fertilization.