“金梗1号”组培快繁技术研究

Study on Tissue Culture and Rapid Propagation of “Jingeng No.1”

目的:采用植物组织培养方法,建立并优化"金梗1号"桔梗新品种的快速繁殖体系。方法:将不同大小的茎尖接种于培养基MS+6-BA 2.0mg/L+IAA0.2mg/L,考察最适宜大小的外植体;以MS培养基为基本培养基,通过不同种类和浓度的植物生长调节剂配比试验,筛选"金梗1号"试管苗最佳快速繁殖与生根培养基。结果:最适宜的外植体为直径0.4mm的茎尖,诱导丛生芽的最适培养基为MS+6-卞基腺嘌呤(6-BA)0.5mg/L+吲哚乙酸(IAA)0.2mg/L+病毒唑(RTCA)2.0mg/L;诱导试管苗生根的最适培养基为1/2 MS+IAA 0.5mg/L+生根粉(ABT)0.2mg/L+多效唑(PP333)0.1mg/L。结论:采用组织培养方法可进行"金梗1号"的快速繁殖,为示范推广奠定了基础。

Objective:To establish and optimize the rapid propagation of Platycodon grandiflorum new variety“Jingeng No.1”by tissue culture.Methods:The shoot tips of different sizes were inoculated on MS medium supplemented with 2.0mg/L 6-BA,and 0.2mg/L IAA to determine the most suitable explants.MS medium being the basal medium,different concentrations,and kinds of plant growth regulators were used to determine the optimal propagation and rooting medium of“Jingeng No.1”by orthogonal tests.Results:The most suitable explants were the shoot tips with the diameter of 0.4 mm.The most appropriate propagation medium was MS medium supplemented with 0.5mg/L 6-BA,0.2mg/L IAA,and 2.0mg/L RTCA,and the most appropriate rooting medium was 1/2MS medium supplemented with 0.5mg/L IAA,0.2mg/L ABT,and 0.1mg/L PP333.Conclusion:Rapid propagation of“Jingeng No.1”could be achieved by tissue culture.The experiment laid a foundation for the demonstration promotion.