基于消食作用优选麦芽适宜芽长

Optimization of Bud Length Based on Digestion Function of Malt

目的研究麦芽发芽过程淀粉酶活力以及对小鼠胃肠运动功能的影响,比较不同芽长麦芽消食作用的差异,优选麦芽最适宜发芽芽长。方法采用3,5-二硝基水杨酸比色法测定不同芽长麦芽中淀粉酶活力;80只KM小鼠随机分为10组,正常对照组、多潘立酮组(6 mg·kg-1)、不同芽长麦芽水煎液组(0.25,0.50,0.75,1.00,1.25,1.50,1.75,2.00cm)(2.28 g·kg-1),每组8只。采用炭末推进实验测定不同芽长麦芽水煎液组小肠推进率以及胃排空率;采用酶联免疫吸附测定(ELISA)法测定各组小鼠血清胃蛋白酶与胃泌素的含量。结果淀粉酶活力随着麦芽芽长的增加呈现先增长后趋平趋势。与正常对照组比较,多潘立酮组显著增加小肠推进率及小肠排空率(P<0.01);不同芽长麦芽水煎液组均能增加小肠推进率以及胃排空率(P<0.01或P<0.05),在麦芽芽长为0.75 cm时最为明显。各不同芽长麦芽组均能增加血清胃蛋白酶与胃泌素含量,未呈现明显规律。结论不同芽长麦芽均能增强肠胃运动,在麦芽芽长达到0.75 cm后效果更显著,建议麦芽发芽芽长应在0.75~1.25 cm。

Objective To study amylase activity and the effect on gastrointestinal motility function in mice during malt germination,and compare the effect of malt with different bud length in promoting digestion to optimize the bud length.Methods The amylase activity in different-length bud of malt was determined by DNS colorimetry.A total of 80 KM mice were randomly divided into ten groups,normal control group,domperidone group(6 mg·kg-1),different-length bud group(0.25,0.50,0.75,1.00,1.25,1.50,1.75,2.00 cm)(2.28 g·kg-1),8 mice in each group.Small intestinal propelling rate and gastric emptying rate in different-length bud of maltdecoctiongroup were determined by the charcoal propulsion experiment.The content of pepsin and gastrin in serum of mice in all groups were determined by enzyme-linked immunosorbent assay(ELISA).Results With the increase bud length of malt,the amylase activity increased at first and then leveled off.Compared with the normal control group,small intestinal propelling rate and gastric emptying rate were significantly increased in domperidone group(P<0.01).Different-length bud of malt decoction could increasesmall intestinal propelling rate and gastric emptying rate(P<0.01 or P<0.05),and the effect of malt was the strongest with bud length of 0.75 cm.The contents of serum pepsin and gastrin were increased in all the malt groups without obvious regularity.Conclusion Different-length bud of malt can enhance gastrointestinal motility function,and it’s most obvious with the bud length of malt reaching 0.75 cm.It is suggested that the sprout bud length of malt should be from 0.75cm to 1.25cm.