羊角槭愈伤组织诱导、增殖与分化

Induction, proliferation, and differentiation of Acer yangjuechi callus

以羊角槭Acer yangjuechi幼嫩茎段和叶片为外植体,研究基本培养基和植物生长调节剂6-苄氨基腺嘌呤(6-BA),萘乙酸(NAA),激动素(KT)和N-苯基-N′^(-1),2,3-噻二唑-5-脲(TDZ)对愈伤组织诱导、增殖与分化的影响。试验通过植物组织培养技术诱导外植体产生愈伤组织,并对愈伤组织进行增殖与分化。结果表明:最适宜愈伤组织诱导的外植体为茎段,基本培养基和适当的植物生长调节剂配比均能促进愈伤组织的诱导增殖与分化;诱导效果最好的基本培养基为木本植物培养基(WPM培养基)。在诱导中, 6-BA的效果显著高于NAA和KT。羊角槭愈伤组织诱导最佳培养基为WPM+0.3 mg·L^(-1)NAA+0.5 mg·L^(-1)KT+0.5 mg·L^(-1)6-BA,诱导率达62.9%;愈伤组织增殖最佳培养基为WPM+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA,增殖倍数达2.4倍。TDZ对分化有重要作用, WPM+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA+1.5 mg·L^(-1)TDZ能促进愈伤组织产生最多不定芽芽点。

To study the effect of basic mediums and plant hormones on callus induction,proliferation,and differentiation with Acer yangjuechi,stems and leaves were used as the explants.Data analysis included variance analysis and poor analysis.Results showed that the optimal explant for callus induction was stems.Basic medium and a proper phytohormone ratio both promoted callus induction,proliferation,and differentiation.The optimum medium for induction was woody plant medium(WPM).The effect of 6-benzylaminopurine(BA)on callus induction was better than 1-naphthaleneacetic acid(NAA)and kinetin(KT).Data analysis of the experimental results showed that the best medium for callus induction was WPM+0.3 mg·L^-1 NAA+0.5 mg·L^-1 KT+0.5 mg·L^-1 6-BA.The best medium for callus proliferation was WPM+1.0 mg·L^-1 6-BA+0.1 mg·L^-1 NAA.Thidiazuron(TDZ)played an important role in differentiation with the medium WPM+1.0 mg·L^-1 6-BA+0.1 mg·L^-1 NAA+1.5 mg·L^-1 TDZ,promoting callus to produce the largest number of adventitious buds.