嗅黏膜神经干细胞对大鼠神经元凋亡的影响

Effect of Olfactory Mucosal Neural Stem Cells on Neuronal Apoptosis in Rats

目的探讨嗅黏膜神经干细胞(OM-NSCs)对大鼠神经元凋亡的影响。方法从雄性Sprague-Dawley大鼠分离提取OM-NSCs,从Sprague-Dawley胎鼠分离神经元。体外培养后,神经元分为空白组、对照组和实验组。空白组正常培养,对照组加白细胞介素-1β(IL-1β)刺激24 h后正常培养,实验组于IL-1β刺激后加OM-NSCs共培养。24 h后使用TUNEL和微管结合蛋白2 (MAP2)抗体双标染色观察神经元凋亡。结果空白组仅有少量细胞凋亡,对照组出现大量凋亡细胞,实验组凋亡细胞数较对照组明显减少(F=39.764,P<0.01)。结论 OM-NSCs能抑制大鼠神经元凋亡,具有神经保护作用。

Objective To investigate the effect of olfactory mucosal neural stem cells(OM-NSCs)on neuronal apoptosis in rats.Methods OM-NSCs were isolated from adult male Sprague-Dawley rats,and neurons were isolated from fetal Sprague-Dawley rats.The neurons were divided into blank group,control group and experiment group after culture in vitro,which were further cultured in nomal,cultured in nomal 24 hours after stimulation of interleukin-1β(IL-1β),and cultured with OM-NSCs 24 hours after stimulation of IL-1β,respectively,for 24 hours.The apoptosis of neurons was observed with double staining of TUNEL and microtubule-associated protein 2 antibodies.Results There were few apoptotic cells in the blank group,and very many in the control group;the apoptotic cells were fewer in the experiment group than in the control group(F=39.764,P<0.01).Conclusion OM-NSCs can significantly inhibit neuronal apoptosis,which may play a role in neuroprotection.