酸马乳源降解亚硝酸盐乳酸菌的分离及鉴定

Isolation and identification of nitrite-degrading lactic acid bacteria from koumiss

为筛选亚硝酸盐降解能力强、产酸快、发酵风味好的乳酸菌,以新疆传统酸马乳为筛选源,经亚硝酸盐降解试验初筛、接种辣椒酱复筛,筛选出一株亚硝酸盐降解率达88.91%、发酵辣椒酱风味佳的乳酸菌C27。总酸复筛试验显示,菌株C27可以明显缩短发酵周期,发酵辣椒酱第8天,总酸含量达2.15 g/100 g。菌株C27经形态观察、生理生化试验及16S rRNA序列分析鉴定为肠膜明串珠菌(Leuconostoc mesenteroides)。耐酸及耐胆盐试验显示,菌株C27在pH 3.0及0.30%胆盐环境中能够生长,满足益生菌在肠胃定植的首要条件。因此菌株C27可以作为发酵辣椒酱专用发酵剂进行生产应用。

In order to screen one lactic acid bacterium with strong nitrite degradation ability,fast acid production rate and good fermentation flavor,using Xinjiang traditional koumiss as screening source,lactic acid bacterium C27 with nitrite degradation rate of 88.91%and good flavor in fermented chili sauce was primary screened by nitrite degradation test and secondary screened by inoculating chili sauce.Secondary screening tests of total acid showed that strain C27 could significantly shorten the fermentation cycle,and the total acid content of the fermented chili sauce was 2.15 g/100 g on the 8 d.Strain C27 was identified as Leuconostoc mesenteroides by morphological observation,physiological and biochemical tests and 16S rRNA sequence analysis.Results of the acid and bile salt tolerance tests showed that strain C27 could grow in the environment with pH 3.0 and bile salt of 0.30%,meeting the primary conditions for probiotic colonization in the intestines and stomach.Therefore,strain C27 could be used as a special fermentation agent for fermented chili sauce and applied for production.