不同品种染色葡萄转色期花色苷积累的差异性研究

Difference of anthocyanins accumulation in different varieties of teinturier grapes at veraison

该研究探究了三个染色葡萄品种烟73、Kolor和Tintorera在转色期其果皮和果肉中花色苷积累的差异。通过利用高效液相色谱-离子阱质谱联用仪(HPLC-ITMS)对花色苷的检测发现,各染色葡萄品种花色苷含量和比例存在组织特异性。果皮和果肉中含量最高的花色苷分别为二甲花翠素-3-O-葡萄糖苷和甲基花青素-3-O-葡糖苷,果皮和果肉中所占比例最高的花色苷分别为3',4',5'-羟基取代花色苷和3',4'-羟基取代花色苷。此外,基于各类花色苷的含量主成分分析(PCA)也能明显区分三个品种的果皮和果肉。就不同品种而言,烟73和Tintorera花色苷组成相似,而与Kolor存在明显差异(P<0.05)。该研究为探究染色葡萄不同组织中花色苷的合成机理提供了基础,并且为染色葡萄在葡萄酒调色中的应用提供了理论依据。

Using Yan73,Kolor and Tintorera varieties teintarier grapes as experimental materials,the differences of anthocyanin accumulation in the peels and pulps of three kinds of teinturier grapes were explored.The contents and proportions of anthocyanins in the peels and pulps of three kinds of teinturier grapes existed tissue specific were detected by anthocyanins by high performance liquid chromatography-ion trap mass spectrometer(HPLC-ITMS).Results showed that the malvidin-3-O-glucoside and peonidin-3-O-glucoside contents were the highest in anthocyanins of the peels and pulps,and the proportions of 3',4',5'-hydroxy-substituted anthocyanin and 3',4'-hydroxy-substituted anthocyanin were the highest in the peels and pulps.In addition,the peels and pulps of three kinds of teinturier grapes could be also clearly distinguished by principle component analysis(PCA)of various anthocyanins contents.For different varieties,Yan73 and Tintorera displayed similar anthocyanin composition,which were significantly different from that of Kolor(P<0.05).This study provided a basis for exploring the synthesis mechanism of anthocyanins in different tissues of teinturier grapes,and provided a theoretical basis for the application of teinturier grapes in wine coloring.