三七总皂苷酶水解产物成分研究

Chemical Constituents from Enzymatic Hydrolysate of Total Saponins of Panax notoginseng

为了研究葡萄糖苷酶催化三七提取物的水解产物中主要皂苷成分。采用色谱法从三七提取物水解产物中分离纯化得到11个皂苷成分。利用波谱解析确定了它们的结构,分别鉴定为20(S)-原人参二醇-20-O-β-D-吡喃木糖基-(1→6)-β-D-吡喃葡萄糖基-(1→6)-β-D-吡喃葡萄糖苷(1),以及10个已知的皂苷成分分别为:人参皂苷compound K(2)、3β,12β,20(S),25-四羟基达玛-23-烯-20-O-β-D-吡喃葡萄糖苷(3)、3β,20(S)-二羟基达玛-24-烯-12β,23β-环氧-20-O-β-D-吡喃葡萄糖苷(4)、3β,12β,20(S)-三羟基-25-过氧羟基达玛-23-烯-20-O-β-D-吡喃葡萄糖苷(5)、人参皂苷F1(6)、人参皂苷Rg1(7)、人参皂苷Rg2(8)、人参皂苷Mc(9)、20(S)-原人参二醇-3-O-β-D-吡喃木糖基-(1→2)-β-D-吡喃葡萄糖基-20-O-β-D-吡喃葡萄糖苷(10)和人参皂苷Re(11)。其中化合物1为新化合物,化合物3~5和10为首次从三七中被分离得到。

This paper presents the saponin constituents from the enzymatic hydrolysate of Panax notoginseng by glucosidase.Eleven saponins were isolated from the hydrolysate of P.notoginseng.On the basis of spectroscopic analysis,their structures were elucidated as 20(S)-protopanaxadiol-20-O-β-D-xylopyranosyl-(1→6)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside(1),ginsenoside compound K(2),3β,12β,20(S),25-tetrahydroxydammar-23-en-20-O-β-D-glucopyranoside(3),3β,20(S)-dihydroxydammar-24-en-12β,23β-epoxy-20-O-β-D-glucopyranoside(4),3β,12β,20(S)-trihydroxy-25-hydroperoxydammar-23-en-20-O-β-D-glucopyranoside(5),ginsenoside F1(6),ginsenoside Rg 1(7),ginsenoside Rg 2(8),ginsenoside Mc(9),20(S)-protopanaxadiol-3-O-β-D-xylopyranosyl-(1→2)-β-D-glucopyranosyl-20-O-β-D-glucopyranoside(10)and ginsenoside Re(11),respectively.Among them,compound 1 was a new saponin.In addition,compounds 3-5 and 10 were isolated from P.notoginseng for the first time.