枯草芽孢杆菌BS-Z15代谢产物抗真菌活性及对小鼠给药的安全性评价

Antifungal Activity of BS-Z15 Metabolites and Its Safety in Mice

本文就枯草芽孢杆菌BS-Z15发酵液粗提抗真菌活性物质的抑真菌谱及对动物的安全性进行了研究。结果表明,除尖孢镰刀菌外,其对大丽轮枝菌、黄曲霉、白色念珠菌等几种有害真菌有拮抗作用,抗菌有效浓度为0. 10 mg/mL;用32. 67 mg/kg粗提活性物质灌胃处理小白鼠60天,未出现中毒症状,且死亡率下降;对小鼠脏器指数、心脏和肝脏组织特征无显著性影响,肝组织抗氧化酶活性显著提高,肝脏MDA含量显著下降。表明BS-Z15抗真菌粗提活性物质对哺乳动物安全无毒,可在动植物病害防治中开发利用。

In this paper,the antifungal spectrum and its safety to animals of crude antifungal from Bacillus subtilis BS-Z15 fermentation broth was studied.The results showed that,with the exception of Fusarium oxysporum,the crude active substance of BS-Z15 had antagonistic effect on several harmful fungi,such as Verticillium dahliae Kleb,Aspergillus flavus,Candida albican and so on,and the effective concentration of crude substance is 0.10 mg/mL.Its safety was evaluated to the crude antifungal,through acute toxicity oral test on model mices by 32.67 mg/kg lavage.And the results showed,no symptoms of poisoning were detected,death rate of the tested mice was reduced,no significant influence on viscera index,heart and liver tissue characteristics.but it was significant that the activity of antioxidant enzymes was increased,the content of MDA was decreased in liver.In conclusion,the crude antifungal extract from BS-Z15 was safe for mammals and has the potential to be used in the prevention and control of fungal diseases in animals and plants.