摘要
核苷酸转移酶(nucleotidyl transferase protein,NTP)能够对RNA 3'末端进行修饰,从而影响其稳定性.通过分析玉米NTP蛋白家族的结构域与拟南芥、水稻中NTP蛋白进化的关系,发现玉米中含有24种NTP基因,并且玉米、拟南芥和水稻3种植物中的NTP基因具有高度同源性与保守性.对玉米NTP基因启动子上游顺式作用元件进行预测分析,结果显示,玉米NTP基因启动子中含有多个与非生物胁迫相关的启动子元件.通过反转录聚合酶链式反应分析在高盐、干旱、植物激素脱落酸的非生物胁迫条件下,玉米NTP相关基因的表达量,研究发现,玉米NTP基因能被非生物胁迫诱导表达.实验表明玉米NTP基因与非生物胁迫相关,且可能参与了植株抵抗逆境胁迫的过程.
Nucleotidyl transferase proteins(NTPs)can modify the 3′ends of small RNA and mRNA and affect their stability.By analyzing the relationship between the domain of NTPs in Zea mays and the evolution of NTPs in Arabidopisis thaliana and Oryza sativa,24 NTPs in Zea mays are identified,and the NTPs in Zea mays,Arabidopsis thaliana,and Oryza sativa are highly homologous and conserved.Sequence analysis shows that cis-acting elements of Zea mays NTP gene promoters contain many prmoter elements related to abiotic stress responses.Further analysis of real-time fluorescence quantitative polymerase chain reaction(RT-PCR)data shows that the expressions of NTP genes in Zea mays are induced by high salinity,drought,and abscisic acid stress,which indicates that NTPs may be involved in plant stress resistance pathway.
作者
陆佳运
宋剑波
王晓彦
莫小为
王碎抗
许欣彤
劳康文
莫蓓莘
LU Jiayun;SONG Jianbo;WANG Xiaoyan;MO Xiaowei;WANG Suikang;XU Xintong;LAO Kangwen;MO Beixin(College of Life and Oceanography,Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen University,Shenzhen 518060,Guangdong Province,P.R.China;College of Science,Jiangxi Agricultural University,Nanchang 330045,Jiangxi Province,P.R.China)
出处
《深圳大学学报（理工版）》
EI
CAS
CSCD
北大核心
2018年第5期536-542,共7页
Journal of Shenzhen University(Science and Engineering)
基金
国家自然科学基金资助项目(31560076)
深圳市科技计划资助项目(JCYJ20170818100038326)。
关键词
核苷酸转移酶
玉米
拟南芥
水稻
顺式作用元件
反转录聚合酶链式反应
非生物胁迫
nucleotidyl transferase protein
Zea mays
Arabidopisis thaliana
Oryza sativa
cis-acting elements
realtime fluorescence quantitative polymerase chain reaction(RT-PCR)
abiotic stress
