甘草硒糖蛋白的制备工艺及对DPPH的清除作用研究

Selenization and Scavenging Activity to DPPH of Licorice Glycoprotein

[目的]考察甘草硒糖蛋白的制备工艺,探究其对DPPH自由基的清除作用。[方法]在硝酸催化剂的作用下加入亚硒酸钠,使甘草糖蛋白与亚硒酸钠作用,生成甘草硒糖蛋白,用HPLC法检测硒含量,以产率和硒含量为指标,确定其合成的最佳工艺。采用分光光度法测定甘草硒糖蛋白、甘草糖蛋白和Vc对DPPH自由基的清除能力。[结果]甘草硒糖蛋白的最佳制备工艺条件:甘草糖蛋白与亚硒酸钠的质量比为1∶1,反应温度为70℃,反应时间为8 h,Ba Cl2的量为1.0 g。在此条件下制备的甘草硒糖蛋白,硒含量平均为4.78 mg/g,平均产率为75.62%。对DPPH自由基的清除能力的试验结果:甘草糖蛋白、甘草硒糖蛋白和Vc的IC50值分别为1.789、1.410和0.427 mg/m L。[结论]该制备方法简单易行,安全,污染较小,硒含量较高;且甘草硒糖蛋白清除DPPH自由基能力强于甘草糖蛋白。

[Objective]The selenization process of glycyrrhiza glycoprotein was investigated and its scavenging effect to DPPH free radical was explored.[Method]Under the action of nitric acid catalyst,adding sodium selenite performed licorice glycoprotein structure modification,generated selenide licorice glycoprotein.Selenium content of organic selenium compounds was detected by HPLC to determine the optimum process,yield and selenium content for indicators.Spectrophotometry was used to determine DPPH free radical scavenging activity of licorice selenium glycoprotein,licorice glycoprotein and Vc.[Result]The optimum process for selenide licorice glycoprotein was reaction temperature of 70℃and time of 8 hours,the mass ratio of icorice glycoprotein to sodium selenite 1∶1,amount of BaCl 2 1.0 g.Under these conditions,the average selenium content was 4.78mg/g,and average extraction rate was 75.62%.Scavenging activity of licorice selenium glycoprotein,licorice glycoprotein and Vc to DPPH radical scavenging ability respectively were 1.789 mg/mL,1.410 mg/mL and 0.427 mg/mL.[Conclusion]Process of glycoprotein selenium was simple,and easy to control,it also polluted less,content of selenium was high.Licorice selenium glycoprotein scavenging DPPH free radicals was stronger than licorice glycoprotein.