甘肃省定西市马铃薯疮痂病新病原Streptomycesgalilaeus的分离、鉴定及生物学特性研究

Isolation,identification and biological characterization of Streptomyces galilaeus,a new pathogen of potato scab in Dingxi City,Gansu Province

对甘肃省定西市安定区的马铃薯疮痂病病原进行了分离、鉴定和生物学特性研究。结果表明,菌株5T-1具有较强致病性,菌落表面呈灰色,有金属光泽,平均直径为4.68mm,可产生黄褐色素,孢子圆形或圆柱形,孢子丝松散,革兰氏染色呈阳性。5T-1的16SrDNA序列与加利利链霉菌Streptomyces galilaeus菌株的相似度为99%,结合形态特征将菌株5T-1鉴定为Streptomyces galilaeus,为甘肃省新报道病原菌。菌株5T-1生长最适温度为30℃,最适光照条件为全黑暗,最适pH为8.5,最佳碳源和氮源分别为肌醇和天冬氨酸。该研究结果为甘肃省马铃薯疮痂病诊断和综合防治提供了依据。

The pathogen of potato scab from Anding District of Dingxi City in Gansu Province was isolated and identified,and its biological characteristics were also determined.The results showed that the strain 5T-1 was highly pathogenic.The colony was gray with a metallic luster and the average diameter of 4.68 mm,which could produce yellow brown pigment.The spores were round or cylindrical and spores silk was loose.Gram staining is positive.The similarity of 16S rDNA sequence of 5T-1 to that of Streptomyces galilaeus was 99%.Combining with the morphological characteristics,the strain 5T-1 was identified as S.galilaeus.It is a newly reported pathogen in Gansu Province.The optimal temperature for strain growth was 30℃.The optimal illumination conditions were darkness and the optimal pH value was 8.5.The best carbon source and nitrogen source were inositol and aspartic acid,respectively.The results provide a basis for the diagnosis and integrated control of potato scab in Gansu Province.