还原氧化石墨烯/硫堇复合物修饰的手性表面对色氨酸的选择性识别

Selective Recognition of Tryptophan by Modified Chiral Surface of Reduced Graphene Oxide/Thionin Complex

制备还原氧化石墨烯/硫堇复合材料,并将其修饰到玻碳电极表面,利用牛血清蛋白分子存在两个活性位点以及手性对映异构体与其作用点不同的特点,将上述修饰电极与牛血清蛋白作用,用于色氨酸(Trp)对映体的选择性识别.采用循环伏安法(CV)及电化学阻抗谱(EIS)对电极的制备及反应过程进行表征.结果表明,在相同实验条件下,该手性表面对色氨酸对映体(D/L-Trp)表现出不同的电化学信号,即该手性表面与D/L-Trp间的反应具有立体选择性,且与L-Trp的作用更强.

We prepared reduced graphene oxide/thionin composite materials,and modified them onto the surface of the glassy carbon electrodes.There were two active sites in bovine serum albumin molecule,and the characteristics of the chiral enantiomers were different from their active points.We immersed the modified electrode in bovine serum albumin,and used for the selective recognition of tryptophan(Trp)enantiomers(D/L-Trp).The preparation and reactive process of the electrode were characterized by cyclic voltammetry(CV)and electrochemical impedance spectroscopy(EIS).The results show that under the sameexperimental conditions,the chiral surface shows different electrochemical signals for D/L-Trp,the reaction between the chiral surface and D/L-Trp has stereoselectivity,and is more effective with L-Trp.