京尼平对胃癌SGC 7901细胞体外增殖、侵袭能力和凋亡的影响及其机制

Influence of genipin in abilities of proliferation and invasion and apoptosis of gastric cancer SGC 7901 cells and their mechanisms in vitro

目的:探讨京尼平(GP)对胃癌SGC 7901细胞的调控作用,并阐明其作用机制点。方法:取对数生长期的SGC 7901细胞,分为对照组和不同浓度(5、10、和20mg·L^(-1))GP组,采用MTT法检测不同时间点(24、48和72h)SGC 7901细胞体外增殖抑制率,应用Transwell小室细胞侵袭实验检测SGC 7901细胞体外侵袭能力,应用Western blotting法检测SGC 7901细胞中Bcl-2、Bax和caspase-3蛋白表达水平。结果:MTT法检测,与对照组比较,作用不同时间后不同浓度GP组SGC 7901细胞增殖抑制率明显升高(P<0.05);Transwell小室细胞侵袭实验,与对照组比较,作用72h后,10和20mg·L^(-1) GP组穿膜细胞数明显降低(P<0.01);Western blotting法检测,与对照组比较,作用72h后,20mg·L^(-1) GP组SGC 7901细胞中Bcl-2蛋白表达水平降低(P<0.01),caspase-3和Bax蛋白表达水平升高(P<0.05)。结论:GP能够抑制SGC 7901细胞的体外增殖和侵袭能力,且能够诱导细胞凋亡,其机制可能与调节Bcl-2、Bax和caspase-3蛋白表达有关。

Objective:To investigate the regulatory effect of genipin(GP)on the gastric cancer SGC 7901 cells,and to clarify its mechanism.Methods:The SGC 7901 cells in logarithmic growth phase were selected and divided into control group and different concentrations(5.0,10.0,and 20.0 mg·L-1)of GP groups.MTT was used to detect the inhibitory rates of proliferation of SGC 7901 cells at different time points(24,48,and 72 h).Transwell chamber cell invasion assay was used to detect the invasion ability of SGC 7901 cells in vitro.The expression levels of Bcl-2,Bax and caspase-3 proteins in the SGC 7901 cells were detected by Western blotting method.Results:The results of MTT assay showed that the inhibitory rates of proliferation of the cells in different concentrations of GP groups after treated with GP for different time were increased significantly compared with control group(P<0.01).The Transwell cell invasion assay results showed that compared with control group,the number of transmembrane cells in 10.0 and 20.0 mg·L-1 GP groups was decreased significantly after treated for 72 h(P<0.01).The results of Western blotting method showed that compared with control group,the expression level of Bcl-2 protein in 20.0 mg·L-1 GP group was decreased after treated for 72 h(P<0.01),and the expression levels of caspase-3 and Bax proteins were increased(P<0.05).Conclusion:GP can inhibit the abilities of proliferation and invasion in vitro of SGC 7901 cells,and induce the apoptosis;its mechanism may be related to the regulation of Bcl-2,caspase-3,and Bax protein expressions.