摘要
目的:探讨纳米载银室温固化型聚甲基丙烯酸甲酯(PMMA)材料对雄性小鼠肝脏组织和肝细胞DNA损伤的影响,评估该纳米复合材料的肝脏毒性。方法:40只6~8周龄雄性昆明小鼠随机分为纳米载银室温固化型PMMA组(72h浸提液、1/2 72h浸提液、1/4 72h浸提液)、室温固化型PMMA组(72h浸提液、1/2 72h浸提液和1/4 72h浸提液)、阴性对照组和阳性对照组(n=5)。以经口灌胃的方式,在实验开始、24h和45h给予除阳性对照剂之外的受试物,阳性对照剂甲磺酸乙酯(EMS)仅在24和45h经口灌胃。阴性对照组小鼠灌胃给予等量生理盐水。最后一次给药3h后摘小鼠眼球取血,行肝脏生化指标检测;处死小鼠后立即完整取出肝脏,计算脏器系数;体内彗星实验(CA)检测尾部DNA百分比(%tail DNA)、尾长(TL)和Olive尾矩(OTM),采用彗星图像分析软件进行图像分析。结果:与阴性对照组比较,各实验组小鼠肝脏系数、丙氨酸氨基转移酶(ALT)和门冬氨酸氨基转移酶(AST)水平比较差异均无统计学意义(P>0.05);与阴性对照组比较,纳米载银室温固化型PMMA组和普通室温固化型PMMA组不同浓度浸提液组尾部%tail DNA、TL和OTM比较差异均无统计学意义(P>0.05)。阳性对照组小鼠%tail DNA、TL和OTM明显高于阴性对照组(t=-40.911,P<0.05;t=-16.620,P<0.05;t=32.943,P<0.05)。相同浓度下纳米载银室温固化型PMMA组%tail DNA、TL和OTM与室温固化型PMMA组比较差异均无统计学意义(P>0.05)。纳米载银室温固化型PMMA组不同浓度浸提液%tail DNA、TL和OTM比较差异无统计学意义(P>0.05)。结论:纳米载银室温固化型PMMA材料未对小鼠肝脏及其功能产生不良影响,彗星实验结果提示其不具有遗传毒性,表明该复合材料具有良好的生物相容性。
Objective:To study the effects of room curing polymethyl methacrylate(PMMA)with nano-silver on the liver tissue and DNA damage of hepatic cells in the mice,and to evaluate the hepatotoxicity of the nanocomposites.Methods:A total of 40 male Kunming mice aged 6-8 weeks were divided into room curing PMMA with nano-silver groups(PMMA-NM groups,72 h extract liquid,1/2 72 h extract liquid,1/4 72 h extract liquid),room curing PMMA groups(PMMA groups,72 h extract liquid,1/2 72 h extract liquid,1/4 72 h extract liquid),negative control group,and positive control group(n=5).All mice were treated by gavage with test compounds at the beginning of experiment and 24 h and 45 h after experiment except the positive control agent,the mice in positive control group were treated with ethylmethylsulfone at 24 and 45 h after experiment,and the mice in negative control group were administrated with the same volume of normal saline by gavage.The eyeball blood of mice was collected for biochemistry analysis of liver 3 h after the final administration.The liver tissue was completely removed immediately after the mice were sacrificed and the organ coefficient was calculated.The percentage of tail DNA(%tail DNA),tail length(TL)and Olive tail moment(OTM)were detected by the in vivo comet assay,and the images were analyzed with analysis software of comet assay.Results:Compared with negative control group,the liver coefficient,the alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels in liver tissue of the mice in various experimental groups had no significant differences(P>0.05);the%tail DNA,TL and OTM in PMMA-NM groups and PMMA groups had no significant differeces(P>0.05).The%tail DNA,TL and OTM of the mice in positive control group were higher than those in negative control group(t=-40.911,P<0.05;t=-16.620,P<0.05;t=32.943,P<0.05).There were no significant differences of the%tail DNA,TL and OTM of the mice between PMMA-NM groups and PMMA groups at the same concentration(P>0.05).There were no significant differences of the%tail DNA,TL and OTM between PMMA-NM groups at different concentrations of extrat liquid(P>0.05).Conclusion:Room curing PMMA with nano-silver doesn’t have adverse effects on the liver and its function in the mice,and the results of comet assay indicates that the material has no genotoxicity,showing that the composite has good biocompatibility.
作者
史金先
张赐童
刘璐
黄天意
闫通通
孙世群
王晓容
SHI Jinxian;ZHANG Citong;LIU Lu;HUANG Tianyi;YAN Tongtong;SUN Shiqun;WANG Xiaorong(Department of Prosthodontics,Stomatology Hospital,Jilin University,Changchun 130021,China;Key Laboratory of Tooth Development and Bone Remodeling and Regeneration,Jilin Province, Changchun 130021, China)
出处
《吉林大学学报(医学版)》
CAS
CSCD
北大核心
2018年第5期962-967,共6页
Journal of Jilin University:Medicine Edition
基金
吉林省发展改革委员会产业创新专项资金项目资助课题(2016C045-2)
吉林省卫计委卫生技术创新项目资助课题(2017J070)