AdipoR2介导C2C12肌管细胞促进3T3-L1前体脂肪细胞的糖代谢能力

Mediation of AdipoR2 in 3T3-L1 preadipocytes co-cultured with C2C12 myotubes for glucose absorption and consumption

[目的]本文旨在探究脂联素受体2(AdipoR2)在C2C12肌管细胞与3T3-L1前体脂肪细胞共培养体系中对3T3-L1前体脂肪细胞葡萄糖吸收与消耗能力的影响。[方法]利用siRNA干扰AdipoR2基因表达,同时利用Transwell细胞培养小室对这2种细胞进行了共培养并检验相关基因的表达水平和细胞葡萄糖消耗能力的变化。[结果]使用siRNA干扰AdipoR2基因的表达后,3T3-L1前体脂肪细胞的AdipoR2基因和葡萄糖转运蛋白基因Slc2a1和Slc2a4表达水平显著下降,3T3-L1前体细胞每单位所消耗的葡萄糖量也显著下降。而在与C2C12细胞共培环境下,3T3-L1前体细胞中AdipoR2基因和葡萄糖转运蛋白Slc2a1、Slc2a4基因表达水平显著上调。同时,3T3-L1前体细胞单位消耗的葡萄糖量显著上升。进一步研究发现,AdipoR2能够有效调节3T3-L1前体脂肪细胞内葡萄糖转运蛋白基因的表达水平与葡萄糖的消耗能力。[结论]在C2C12肌管细胞共培体系中,AdipoR2能够加速3T3-L1前体脂肪细胞对葡萄糖的吸收。

[Objectives]This study was aimed to investigate the mediate capacity of AdipoR 2 gene in 3T3-L1 preadipocytes co-cultured with C2C12 myotubes for the glucose absorption and consumption.[Methods]To testify the gene expression levels and glucose consumption in this co-culture system,we conducted siRNA to suppress the gene expression and Transwell inserts which allowing both of these two cells culturing in the same circumstance.[Results]Our data suggested that since the siRNA treatment,the 3T3-L1 preadipocytes significantly declined the expression of AdipoR 2 gene,subsequently accompanied by the significant decrease of gene Slc 2a1 and Slc 2a4 expression.The glucose consumption test suggested that this capacity was in a lower degree compared to the control group as well.Additionally,in co-culture system AdipoR2 and glucose transport gene were higher significantly expressed resulting in the increment of the glucose content per protein unit.This way indicated that AdipoR 2 gene in 3T3-L1 preadipocytes could regulate the glucose transport gene Slc 2a1 and Slc 2a4 expression and affect the glucose consumption ability.[Conclusions]In summary,to cope with the co-culture influence brought by C2C12 myotubes,AdipoR2 would like to significantly enhance the glucose consumption ability in 3T3-L1 preadipocytes.