LC-MS/MS法快速测定头发中可卡因及其代谢物苯甲酰爱康宁

Rapid Determination of Cocaine and Its Metabolite Benzoylecgonine in Hair by LC-MS/MS

目的建立头发中可卡因(cocaine)及其代谢物苯甲酰爱康宁(benzoylecgonine)的液相色谱-串联质谱(LC-MS/MS)快速测定方法。方法去污处理后的头发中加入氘代内标(可卡因-D_3和苯甲酰爱康宁-D_8),经甲醇超声提取后用Restek Allure PFP丙基柱分离,采用多反应监测模式同时测定可卡因和苯甲酰爱康宁。结果头发中可卡因和苯甲酰爱康宁在0.02~10.00 ng/mg质量分数范围内线性关系良好,检出限均为0.01 ng/mg。结论本研究所建方法样品前处理简单、快速、选择性好,适用于头发中可卡因及代谢物苯甲酰爱康宁的检测。

Objective To establish a rapid determination method with LC-MS/MS for cocaine and its metabolite benzoylecgonine in hair.Methods Deuterated internal standards(cocaine-D3 and benzoylecgonine-D8)were added to the decontaminated hair.After the extraction by ultrasonication with methanol,the compounds were separated by the Restek Allure PFP propyl column,and cocaine and benzoylecgonine were simultaneously analysed in multiple reaction monitoring mode.Results The cocaine and benzoylecgonine in hair showed a good linearity in the range of mass fraction between 0.02 and 10.00 ng/mg with the limits of detection of 0.01 ng/mg.Conclusion The developed method is simple and rapid with a good selectivity,which is suitable for the determination of cocaine and its metabolite benzoylecgonine in hair.