摘要
合成了3种水溶性铜卟啉配合物,分别为水溶性含溴铜卟啉(CuP-1)及其溴取代衍生物(CuP-2、CuP-3),并采用核磁氢谱、元素分析、质谱等手段进行了结构表征。利用紫外光谱法、EB-DNA荧光淬灭法、粘度法以及圆二色谱法等光谱法研究了CuP-1、CuP-2和CuP-3与小牛胸腺DNA(CT-DNA)的相互作用。采用噻唑兰(MTT)法,以体外培养的宫颈癌细胞(Hela)和乳腺癌细胞(MDA)为测试细胞株对CuP-1~CuP-3的抗肿瘤活性进行检测。人成纤维细胞(L929)作为正常细胞系。光谱法实验结果表明,CuP-1以插入的方式与CT-DNA相互作用,而CuP-2和CuP-3与CT-DNA以沟面结合的方式相互作用,且CuP-1与CT-DNA的结合能力优于CuP-2和CuP-3。体外抗癌活性实验结果表明,CuP-1~CuP-3对Hela和MDA均有体外抑制细胞增殖作用,呈时间、剂量依赖关系,并且CuP-1的体外抗肿瘤活性明显优于CuP-2、CuP-3。
Three water-soluble copper porphyrin complexes(water-soluble bromine-containing copper porphyrin CuP-1 and its bromine substituted derivatives CuP-2 and CuP-3)have been synthesized and characterized by 1H NMR,elementary analysis and MS spectra.The interaction of CuP-1,CuP-2 and CuP-3 with CT-DNA have been investigated by UV spectrum,EB-DNA fluorescence quenching,viscosity method and circular dichroism spectrum.Moreover,the antitumor activities of CuP-1~CuP-3 against Hela(cervical cancer)cell lines and MDA(breast carcinoma)cell lines have been studied by MTT assay.L929(human fibroblast)cell line was used as a normal cell lines.Various spectroscopic approaches indicates that CuP-1 could effectively bind with CT-DNA through intercalation mode,while CuP-2 and CuP-3 may interact with CT-DNA by outside groove binding mode.And the binding affinity of CuP-1 with CT-DNA is better than its derivatives CuP-2 and CuP-3.In vitro antitumor activity experiments showed that CuP-1~CuP-3 can inhibit the growth of Hela and MDA cell lines,and the inhibition shows concentration and time dependent.In addition,antitumor activity in vitro of CuP-1 is much better than CuP-2 and CuP-3.
作者
哈斯其美格
陈丽华
肖朝虎
HASI Qi-Mei-Ge;CHEN Li-Hua;XIAO Chao-Hu(Experimental Center,Northwest Minzu University,Lanzhou 730070,China)
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
2018年第10期1841-1850,共10页
Chinese Journal of Inorganic Chemistry
基金
甘肃省高等学校科研项目(No.2017B-87)
西北民族大学"一优三特"学科中央高校基本科研业务费(No.31920170168)资助