PD-1、FOXP3和CSF-1R蛋白表达对霍奇金淋巴瘤患者预后的影响

Effect of PD-1,FOXP3 and CSF4R Protein Expression on the Prognosis of Patients with Hodgkin's Lymphoma

目的:研究集落刺激因子1受体(CSF-1R)、叉头转录因子3(FOXP3)和程序性死亡1(PD-1)蛋白表达对霍奇金淋巴瘤患者预后的影响。方法:记录本院收治的54例霍奇金淋巴瘤患者的临床特征资料和治疗方案。采集患者标本,采用免疫组织化学染色法检测微环境相关的预后因子CSF-1R、FOXP3及PD-1蛋白表达,同时通过原位杂交技术检测EB病毒及其小编码的mRNA(EBER);探讨CSF-1R、FOXP3及PD-1 3种蛋白表达与霍奇金淋巴瘤患者预后的关系,并采用单因素分析和多因素分析(Cox比例风险模型)研究影响霍奇金淋巴瘤患者预后的相关因素。结果:54例霍奇金淋巴瘤患者中,CSF-1R^+22例(40. 74%),FOXP3高表达28例(51. 85%),PD-1^+9例(16. 67%)。单因素分析结果发现,国际预后指数(IPI)评分、EBER和FOXP3蛋白表达是霍奇金淋巴瘤患者无进展生存(PFS)的影响因素(均P <0. 05);临床分期(Ann Arbor分期)、IPI评分、EBER、CSF-1R和FOXP3蛋白表达是患者总生存(OS)的影响因素(均P <0. 05)。多因素分析(Cox比例风险模型)结果发现,EBER状态是霍奇金淋巴瘤患者PFS、OS的影响因素(P <0. 05); FOXP3蛋白表达是患者PFS的影响因素(P <0. 05); Ann Arbor分期、CSF-1R蛋白表达均是患者OS的影响因素(均P <0. 05)。结论:CSF-1R、FOXP3与霍奇金淋巴瘤患者的预后有密切联系,可为该病的靶向治疗提供一定的依据。

Objective:To investigate the effect of the expression of colony-stimulating factor 1 receptor(CSF-1R),forkhead box protein 3(FOXP3)and programmed death 1(PD-1)protein on the prognosis of patients with Hodgkin's lymphoma.Methods:The clinical features and treatment schemes of 54 patients with Hodgkin's lymphoma treated in ou hospital were recorded,the specimens were collected and the relevant micro-environment prognostic factors such as the protein expression of CSF-1R,FOXP3 and PD-1 were detected by immunohistochemical staining,and EB virus and EB virus-encoded RNA(EBER)were detected by in situ hybridization;the correlation bet\veen CSF-1 R,FOXP3,PD-1 protein expression with prognosis of patients with Hodgkin lymphoma were analyzed;both the unvaiate analysis and multivariate analysis(using Cox proportional hazard model)were uased to analyze the influence factors of prognosis.Results:Among the 54 cases of Hodgkin's lymphoma,22 cases(40.74%)were positive for CSF-1R,21 cases(51.15%)showed high expression of FOXP3 and 9 cases(12.27%)were positive for PD-.Unvariate analysis revealed that the international prognostic index(IP、)score,EBER and FOXP3 protein expression were the effecting factors of progression-free survival(PFS)of patients with Hodgkin's lymphoma(all P<0.05);clinical staging(Ann Arbor stage),IPI score,EBER,CSF-1R and FOXP3 protein expression were the effecting factors of overall survival(OS)(all P<0.05).Multivariate analysis(Cox proportional hazard model)results showed that the EBER state was the effecting factors of PFS and OS in patients with Hodgkin's lymphoma(P<0.05);the expression of FOXP3 protein was the effecting factors of PFS(P<0.05);Ann Abor stage and the expression of CSF-1R protein were the effecting factors for OS(both P<0.05).Conclusion:CSF-1R and FOXP3 closely relate with the prognosis of the patients with Hodgkin's lymphoma,which can provide a basis for targeted therapy of this disease.