Blimp-1表达低下与再生障碍性贫血发病的相关性研究

Relationship of Blimp-1 Hypoexpression with Pathogenesis of Aplastic Anemia

目的:研究Blimp-1表达低下与Treg数量异常及和再生障碍性贫血(AA)发病的关系。方法:以鼠源性IFN-γ联合白消安的方法建立AA小鼠模型,在不同建模天数取材并检测脾脏Treg数量,分选Treg并检测Prdm1的表达水平。结果:AA组小鼠的Treg数量较对照组低,且Treg减少程度与AA病情呈正相关(r=0.805);Prdm1表达水平越高,Treg/淋巴细胞比例越高,二者间存在正相关关系(r=0.543)。结论:Blimp-1表达水平低下通过降低Treg增殖程度和减少Treg数量而介导AA发病和病情进展。

Objective:To study the relationship of Blimp-1 hypoexpression with abnormality of Treg level and pathogenesis of aplastic anemia(AA).Methods:The mouse model with AA was established by adminis tration of IFN-γcombined with busulfan.The samples were collected at different day establishing AA model,and the spleen Treg number was detected,the Treg cells were sorted and expression level of prdm-1 was detected.Results:The number of Tregs in mice with AA was lower than that in control mice,moreover,the level of Treg decrease positively correlated with the AA severity(r=0.805),the higher the expression level of prdm-1,the higher the ratio of Treg/lymphocytes,showing positive correlation between them(r=0.548).Conclusion:Blimp-1 expression may promote the proliferation and differentiation of Treg.The hypoexpression of Blimp-1 mediates the pathogenesis of AA and promotes progression of AA through reducing the proliferation of Treg,and decreacing the number of Treg.