Th17细胞、IL-17和MMP-13在过敏性紫癜发病机制中的作用

Role of Th17 Cells, Interleukin-17 and Matrix Metalloproteinase-13 in Pathogenesis of Henoch-Schonlein Purpura

目的:通过测定过敏性紫癜(Henoch-Schonlein purpura,HSP)患者急性期外周血中Th17细胞、IL-17、基质金属蛋白酶-13(matrix metalloproteinase-13,MMP-13)的水平以及患者皮损处IL-17的表达,探讨HSP的免疫学发病机制。方法:应用流式细胞术(Flow Cytometry,FCM)检测HSP组和健康对照组外周血中Th17细胞比例;双抗体夹心酶联免疫吸附(ELISA)法检测上述研究对象血浆中IL-17和MMP-13的浓度;免疫组织化学法检测HSP组皮损组织和健康对照组皮肤组织IL-17的表达情况。结果:HSP组急性期外周血中Th17细胞比例为(1.21±0.59)%,明显高于对照组的(0.71±0.26)%(t=4.907,P <0.01);HSP组急性期血浆中IL-17和MMP-13浓度分别为(64.58±36.21)pg/ml和(17.57±5.40)ng/ml,均明显高于对照组的(26.16±14.90)pg/ml和(11.55±4.40)ng/ml(t=6.183,P <0.01和t=5.022,P <0.01);HSP组皮损组织中IL-17的免疫组织化学积分光密度值为(7.26±2.34),非常显著高于对照组的(4.61±1.82)(t=2.877,P <0.01)。结论:Th17细胞、IL-17以及MMP-13有可能参与过敏性紫癜的发病过程。

Objective:To explore the immunopathogenesis of Henoch-Schonlein purpara(HSP)by detecting the levels of Th17 cells,IL-17 and matrix metallo proteinase-13(MMP-13)in peripheral blood and the expression of IL-17 in skin lesions at acate phase of HSP.Methods:Th17 cell ratio in peripheral blood of HSP group and healthy control group was defected by flow cytometry,the plasma levels of IL-17 and MMP-13 in HSP group and healthy control group were defected by ELISA,and expression level of IL-17 in skin lesion of HSP group and skin tissue of healthy control group was deternined by:immunohistochemistry method.Results:the ratio of Th17 cells in periphral blood of HSP group(1.21±0.59%)was very signif cantly higher than that in peripheral blood of control group(0.71±0.26%)(t=4.907,P<0.01).The plasma levels of IL-17 and MMP-13 at acute phase of HSP were very significantly higher than those in control group(64.58±36.21)pg/ml vs(26.16±14.90)pg/ml and(17.57±5.40)pg/ml vs(11.53±4.40)pg/ml respectively(t=6.183,P<0.01 and t=5.022,P<0.01).The integrool optical density of IL-17 in skin lesin tissue of HSP group(7.26±2.34)was higher than in control group(4.61±1.82)(t=2.877,P<0.01).Conclusion:Th17 cells,IL-17 and MMP-13 may be involved in the immunological pathogenesis of HSP.