IL-12B基因rs3212227位点多态性与川崎病及其并发冠状动脉损伤的关联性研究

Relationship between locus rs3212227 of IL-12B gene polymorphism and Kawasaki disease and that complicated with cornary artery lesion

目的:探讨IL-12B基因rs3212227位点多态性与川崎病(KD)及其并发冠状动脉损伤(CAL)的关联性。方法:收集2004年至2014年间83例KD患儿及86例健康儿童外周血标本,提取DNA,应用基因扩增-聚合酶链限制性长片段法(PCR-RFLP)检测IL-12B基因rs3212227位点多态性,并用直接测序法进行验证;χ~2检验分析该位点多态性与KD及其并发CAL之间是否存在关联性,P<0. 05代表差异有统计学意义。结果:KD组AA、AC、CC基因型分布和A、C等位基因分布与对照组比较差异无统计学意义(χ~2=4. 095、3. 31,P> 0. 05)。KD组中合并CAL组基因型和等位基因分布与冠状动脉正常组(NCAL组)比较差异亦无统计学意义(χ~2=1. 586、1. 254,P>0. 05)。结论:IL-12B基因rs3212227位点多态性与川崎病及其并发冠状动脉损伤有关联。

Objective:To study the relationship between the locus rs3212227 of IL-12B gene polymorphism and Kawasaki disease(KD)and that complicated with coronary artery lesion(CAL).Methods:83 cases of patients with KD(KD group)and 86 healthy children as controls(control group)were studied from 2004 to 2014.Polymorphism analysis of the locus rs3212227 of IL-12B gene was detected by polymerase chain reaction-restriction fragment length polymorphism method(PCR-RFLP).Then direct sequencing was used to verify the accuracy.Chi-square test was used to analyze whether there was a correlation between the locus rs3212227 of IL-12B gene polymorphism and Kawasaki′s disease and that with coronary artery lesion(CAL).Results:There were no significant differences in genotype frequency of AA,AC,CC and allele frequency of A,C between KD group and healthy controls(χ2=4.095,3.31,respectively,both P>0.05).Neither the genotype nor allele frequency was observed significant different between KD complicated with CAL(CAL group)and without CAL(NCAL group)(χ2=1.586,1.254,respectively,both P>0.05).Conclusion:The association was not found between the polymorphism of locus rs3212227 of IL-12B gene with Kawasaki Disease(KD)and that complicated with CAL in this study.