摘要
目的探讨Klotho基因G-395A位点多态性与糖尿病肾病的关系。方法选取单纯2型糖尿病患者147例(单纯糖尿病组)、糖尿病肾病患者98例(糖尿病肾病组)、健康志愿者100例(健康组),采用实时荧光定量聚合酶链式反应技术检测各组Klotho基因G-395A位点多态性,采用多因素Logistic回归分析法分析糖尿病肾病发病的危险因素。结果经实时荧光定量聚合酶链式反应技术检测,Klotho基因G-395A位点出现了AA、GA、GG三种基因型。健康组与单纯糖尿病组AA基因型分布明显低于糖尿病肾病组,GA基因型+GG基因型分布明显高于糖尿病肾病组(P均<0. 05);健康组与单纯糖尿病组AA基因型、GA基因型+GG基因型分布比较P均>0. 05。健康组与单纯糖尿病组A等位基因频数明显低于糖尿病肾病组,G等位基因频数明显高于糖尿病肾病组(P均<0. 05);健康组与单纯糖尿病组组A、G等位基因频数比较P均>0. 05。多因素Logistic回归分析显示,Klotho基因G-395A位点AA基因型分布增加是糖尿病肾病发病的危险因素(OR=2. 026,95%CI:0. 902~4. 552,P=0. 087)。结论 Klotho基因G-395A位点多态性与糖尿病肾病发病有关,该位点AA基因型分布增加可能是糖尿病肾病发病的独立危险因素。
Objective To explore the relationship between polymorphism of the Klotho gene G-395A and the diabetic nephropathy(DN).Methods Totally 245 patients were selected,including 147 diabetic patients(single diabetic group),98 DN patients(DN group),and 100 healthy volunteers(control group),the Klotho gene G-395A genotype and allele were detected by real-time fluorescent quantitative PCR.Multivariate Logistic regression analysis was used to analyze the risk factors of DN.Results Three genotypes,AA,GA,and GG,were found at the G-395A site of Klotho gene by re-al-time fluorescent quantitative PCR.The distribution of AA genotype in the control group and single diabetic group was significantly lower than that in the DN group(P<0.05),and the distribution of GA+GG genotype was significantly higher than that of the DN group(P<0.05).No significant difference was found in the distribution of AA and GA+GG geno-types between the control group and the single diabetic group(P>0.05).The frequency of allele A in the control group and single diabetic group was significantly lower than that in the DN group,and the frequency of allele G was significantly higher than that in the DN group(both P<0.05).There was no difference in the frequencies of alleles of A and G be-tween the control group and the single diabetic group(P>0.05).Multiple Logistic regression analysis showed that Klotho gene G-395A site AA was a risk factor for DN(OR=2.026,95%C/:0.902^.552,P=0.087).Conclusion The poly-morphism of the Klotho gene G-395A site is related to the incidence of DN,and the distribution of AA genotype may be an independent risk factor for the incidence of DN.
作者
李杨
黄邓高
郑琳麟
曹卉
高元慧
范忠诚
LI Yang;HUANG Denggao;ZHENG Linlin;CAO Hui;GAO Yuanhui;FAN Zhongcheng(Haikou Hospital Affiliated to Xiangya Medical collge of central South University,Haikou 570203,China)
出处
《山东医药》
CAS
2018年第36期5-8,共4页
Shandong Medical Journal
基金
海南省卫生厅医学科研课题资助项目(琼卫2013资助-012号)