摘要
目的探讨CCAT增强子结合蛋白α(C/EBP-α)在肝星状细胞(HSCs)自噬过程中是否发挥作用。方法构建携带C/EBP-α的慢病毒载体Lenti-C/EBP-α,感染大鼠肝星状细胞株HSC-T6,Western blot检测自噬相关蛋白LC3 (微管相关蛋白1轻链3)表达;采用自噬激活剂Rapamycin处理HSC-T6,Western blot检测C/EBP-α和LC3的表达;将HSC-T6经Lenti-C/EBP-α及自噬激活剂Rapamycin共处理,Western blot检测C/EBP-α和LC3的改变。结果 HSC-T6感染Lenti-C/EBP-α后,自噬活性降低,LC3表达下降;Rapamycin处理HSC-T6后,自噬活性增高,C/EBP-α表达下降,LC3表达增多;HSC-T6经Lenti-C/EBP-α及自噬激活剂Rapamycin共处理,与单纯使用Rapamycin比较,C/EBP-α表达升高,LC3激活减少,抑制HSCs激活。结论 C/EBP-α可以抑制自噬激活,从而抑制HSCs激活,为C/EBP-α抗肝纤维化提出了新思路。
Objective To investigate the role of CCAT enhancer binding proteinα(C/EBP-α)in autophagy of hepatic stellate cells(HSCs).Methods C/EBP-αLentiviral vector was constructed.HSC-T6 was treated with C/EBP-αLentiviral vector,autophagy activator Rapamycin or C/EBP-αLentiviral vector plus Rapamycin.Autophagyrelated protein LC3 and C/EBP-αwere detected with Western blotting.Results Treatment of Lenti-C/EBP-αinduced decreased expression of LC3 in HSC-T6 while Rapamycin upregulated levels of LC3 and downregulated expression of C/EBP-α.Co-incubation of Lenti-C/EBP-αand Rapamycin suggested that C/EBP-αexpression was increased and LC3 expression was decreased when compared with Rapamycin only group.Conclusion C/EBP-αinhibits activation of HSC-T6 inhibition of autophagy,which may be a novel approach for anti-hepatic fibrosis.
作者
陈耀丽
翟颖真
刘桓余
彭继英
尹为华
陶丽丽
刘秀萍
Yao-li Chen;Ying-zhen Zhai;Huan-yu Liu;Ji-ying Peng;Wei-hua Yin;Li-li Tao;Xiu-ping Liu(Department of Pathology,Peking University Shenzhen Hospital,Shenzhen,Guangdong 518036,China;Shanghai Ackerman Medical Laboratory,Shanghai 200032,China;Department of Pathology,School of Basic Medical Sciences,Fudan University,Shanghai 200331,China)
出处
《中国现代医学杂志》
CAS
2018年第27期1-4,共4页
China Journal of Modern Medicine
基金
北京大学深圳医院课题(No:JCYJ2017010)
广东省自然科学基金博士科研启动项目(No:2015A030310031)
