白介素21在42日龄中国黄羽鹌鹑盲肠扁桃体中的定位

Immunohistochemical localization of interleukin-21 in cecal tonsil of 42 day old Chinese Yellow Feather quail

为探明白介素21(IL-21)在鹌鹑机体免疫调节的作用机制,观察IL-21的分布特点,取42日龄中国黄羽鹌鹑盲肠扁桃体,运用免疫组织化学法对白介素21进行组织学定位,观察阳性细胞的形态及分布情况。结果显示,IL-21阳性反应细胞多呈圆形或椭圆形,少数呈梭形,有的呈团块状,阳性反应细胞主要分布于黏膜上皮下固有层中,少数位于肠腺周围,生发中心周围也有少量阳性细胞分布。证实IL-21在鹌鹑盲肠扁桃体中广泛存在,分布数量及反应强度与局部免疫细胞及机体免疫状态有关。

To investigate the mechanism of interleukin-21 in the immune regulation of the Chinese Yellow Feather quail body,the distribution characteristics of IL-21 were observed.The quail tonsils of 42 day old Chinese Yellow Feather quail were taken,immunohistochemical SABC method was used for histological localization of interleukin-21 in the tonsil,and the morphology and distribution of the IL-21positive cells were observed.The results showed that the IL-21 positive reaction cells were round or elliptic,and a few were fusiform,some were clumpy.The positive reaction cells were mainly distributed in the lamina propria of the underlying layer of the epithelium of cecal tonsil,and a few were around the intestinal gland.There was also a small amount of IL-21 positive cells around the germinal centers.The results confirmed that IL-21 was widely distributed in the cecal tonsil of quail.The distribution and reaction strength of IL-21 positive cells were related to the local immune cells and the immune state of the body.