自然老化谷类种子的二维红外光谱研究

Discrimination of Grain Seeds of Natural Aging by Two-Dimensional Infrared Spectroscopy

种粮老化影响种子活力及粮食品质。利用傅里叶变换红外光谱(FT-IR),二阶导数红外光谱(SDIR)和二维相关红外光谱(2D-IR)方法对不同储藏年份的大麦,小麦,稻谷,玉米和高粱种子进行区分研究。结果显示,原始光谱中几个峰强比有差异;在二阶导数红外光谱中,不同储藏年份种子在1 800~800cm^(-1)范围内的吸收峰强度和形状显示出差异。二维相关红外光谱结果显示:大麦在1 350~1 800cm^(-1),小麦,稻谷,玉米和高粱在860~1 690cm^(-1)范围内有明显差异,随储藏年份增加,自动峰和交叉峰的数目减少,强度减弱。结果表明,应用傅里叶变换红外光谱结合二维相关红外光谱能有效快速地区分不同储藏年份的谷类种子。

Seed aging affects seed vigor and the quality of seeds as food.In this paper,barley,wheat,rice,corn and sorghum seeds of different storage years werediscriminated by Fourier transform infrared(FT-IR)spectroscopy combined withsecond derivative infrared(SD-IR)spectroscopy and two-dimensional correlation infrared(2D-IR)spectroscopy.The results showed that several peak intensity ratios in the original spectra were different.In addition,the SD-IR spectra showed differences concerned with peak shape and intensity particularly in the range of 1 800~800 cm-1.Besides,the 2D-IR spectra showed that the number and intensitiesof auto-peaks and cross-peaks have clear differences in the range of 1 350~1800 cm-1 for barley,and in the range of 860~1 690 cm-1 for wheat,rice,corn and sorghum.It is demonstrated that FT-IR and 2D-IR spectroscopy could be used to discriminate grain seeds of different storage years rapidlyand effectively.