同步与三维荧光光谱法研究日落黄和人血清蛋白的相互作用

Application of Synchronous and Three-Dimensional Fluorescent Spectrometry in Studies on Interaction Between Sunset Yellow and Human Serum Albumin

日落黄是一种具有潜在危害性的人工合成色素。选用人血清蛋白为研究对象,利用荧光光谱法分析了日落黄对人血清蛋白荧光的猝灭作用,确定了荧光猝灭反应的猝灭常数Ksv,结果表明日落黄对人血清蛋白的荧光猝灭作用类型为静态猝灭。运用同步荧光光谱法分析日落黄与人血清蛋白结合表明:日落黄与人血清蛋白的结合靠近色氨酸附近,引发色氨酸残基趋向伸展状态,人血清蛋白的结构发生改变。此外,在模拟人体生理条件下选用10种不同的金属离子加入到日落黄和人血清蛋白的反应体系中,运用三维荧光法检测金属离子对体系的影响,结果表明Cu^(2+),Pb^(2+),Ni ^(2+)和Mn^(2+)对猝灭过程有着促进作用,其中Ni ^(2+)的影响最大,增幅达22.6%;而Fe^(2+)和Zn^(2+)对猝灭过程有着抑制作用,抑制率达14.12%和14.2%。本研究的实验方法适用于研究食品添加剂的毒性,有助于保护食品安全,维护人体健康。

Sunset Yellow(SY)is a potentially harmful artificial synthetic pigment.Thispaper,taking human serum albumin(HSA)as the research object,analyzes the quenching effect of SY on HSA and determines the quenching constant Ksv of the quenching reaction with the fluorescence spectroscopy method.The results show that the quenching effect of SY on HSA is static quenching.In addition,based on the analysisof the binding of SY and HSA with the synchronous fluorescence spectroscopy method,it is found that the binding of SY and HSA is near tryptophan,whose residue is caused to be in a stretching state and the structure of HSA has changed.Furthermore,this paper selects ten types of metal ion,adds them into the reaction system of SY and HSA under the simulated human physiological conditions,and detects the impacts of the metal ions on the system with the three-dimensional fluorescent spectrometry method,which demonstrating that Cu 2+,Pb2+,Ni 2+and Mn 2+exert facilitation effects on the quenchingprocess,and Ni 2+shows the largest impacts,with an increase of 22.6%,while Fe 2+and Zn 2+have inhibition effects on the quenching process,with an inhibition ratio of 14.12%and 14.2%,respectively.Therefore,the experimental method is applicable to the study on the toxicity of food additives and conducive to ensureing food safety as well as maintaining human health.