甘肃省永靖县奶牛布鲁氏菌病监测与病原分离鉴定

Surveillance,Isolation and Identification of Brucella Surveillence in Cows in Yongjing County of Gansu Province

为查明甘肃省永靖县奶牛布鲁氏菌病流行情况、感染菌种和类型,2013—2017年对永靖县8月龄以上奶牛进行了布鲁氏菌病监测,对2016年检出的3份疑似感染布鲁氏菌奶牛的脾脏进行了病原分离与鉴定。结果显示:2013—2014年检测奶牛444头,未检出阳性;2015—2016年检测奶牛706头,检出阳性63头,个体阳性率为8.92%;3份脾脏样本均为布鲁氏菌PCR检测阳性,细菌分离培养15 d,只有1份生长菌落;将分离菌株用AMOS-PCR进行检测,获得流产布鲁氏菌特征的扩增条带,且分离菌株的omp25基因测序结果与流产布鲁氏菌高度吻合。本监测和细菌分离鉴定结果为该地布鲁氏菌病防控提供了技术支撑。

In order to find out the prevalence status of cow brucellosis in Yongjing County of Gansu Province and to determine the types of infection strain,during 2013 to 2017,brucellosis surveillance was carried out towards cows over 8 months of age,and 3 spleen samples were detected Brucella-positive in 2016.Then isolation and identification of Brucella in suspected samples was carried out.Results showed that during 2013 to 2014,444 cows were tested,no positive cow was found;during 2015 to 2016,706 cows were tested,and 63 of them were detected brucellosispositive,the individual positive rate was 8.92%.For the 3 suspected spleen samples,PCR tests all showed positive results.After 15 days of bacteria isolation and culture,bacteria colonies only grew in one culture plate.At last,one bacterial strain was isolated.Through AMOS-PCR detection,the amplified band revealed the characteristic of Brucella abortus,also,the sequencing results of omp25 gene of the isolate showed high consistency with that of Brucella abortus.In conclusion,the research results would provide technical supports for brucellosis prevention and control in Yongjing County.