犊牛魏氏梭菌的分离鉴定及致病性研究

Isolation,Identification and Pathogenicity Analysis of Clostridium perfringens from Calf

2017年1月,山东省某规模化奶牛场10～20日龄犊牛出现腹泻、拉血便、呼吸困难等症状,部分犊牛突然死亡。通过流行病学调查和尸体剖检,初步怀疑为魏氏梭菌感染。采集病料进行病理组织学检查、细菌分离鉴定、生化试验、16S及毒素基因检测等,证明疫情由A型魏氏梭菌所致。对死亡犊牛的肠内容物及分离菌株进行致病性研究,发现肠内容物及分离菌株具有较强的致病性。药敏试验结果显示,该菌对青霉素、头孢噻呋、恩诺沙星等表现极敏感。根据病原确诊情况及药敏试验结果,指导临床用药,及时控制了牛场疫情。

In January 2017,some calves between the age of 10 to 20 days suffered from diarrhea,bloody stools,dyspnea and other symptoms in a large-scale dairy farm in Shandong Province,some of them died suddenly.According to epidemiological investigation and autopsy,Clostridium perfringens was initially suspected as the cause.Then related samples were collected and a series of tests were conducted.Based on histopathologic examination,isolation and identification of bacteria,biochemical test and 16s and toxin gene detection,the cause of this epidemic was finally confirmed as type A Clostridium perfringens infection.The pathogenicity of the isolated strain and intestinal contents from dead calves were analyzed,results showed that both of them had relatively strong pathogenicity.Besides,drug sensitivity tests showed the isolated Clostridium perfringens was extremely sensitive to Penicillin,Cefotifuran,Enoxacin,etc.After reasonable administration of clinical drugs,the epidemic was controlled timely.