小鼠肾发育中Slit2及其受体Robo1的表达

Expressions of Slit2 and its receptor Robo1 during development of mouse kidneys

目的观察并检测Slit2及其受体Robo1在小鼠肾发育中的表达变化。方法通过免疫组织化学技术系统观察小鼠肾脏Slit2和Robo1的表达和定位;应用蛋白印迹技术检测小鼠肾脏Slit2和Robo1表达的变化规律。结果 Slit2和Robo1在胚龄12 d小鼠肾脏生肾区开始表达;Slit2在小鼠输尿管芽及其周围的间充质聚集的部位阳性表达,Robo1在小鼠肾脏生肾区广泛表达,主要分布于生后肾组织和输尿管芽上皮细胞基底面;在肾单位发育过程中,Slit2定位表达于逗号小体阶段和S小体阶段,且表达较强,在Ⅲ期肾小体阶段、Ⅳ期肾小体阶段及成熟肾小体阶段有微弱表达;Robo1定位表达于逗号小体阶段、S小体阶段、Ⅲ和Ⅳ期肾小体阶段,且表达较强,在成熟肾小体阶段有微弱表达;Slit2及其受体Robo1在肾脏泌尿小管如近端小管、远端小管及集合管发育过程中均有表达。蛋白印迹检测从小鼠胚龄14 d开始,Slit2表达先递增,至胚龄16 d达峰值,而后逐渐递减。Robo1表达从小鼠胚龄14 d开始逐渐递减。结论 Slit2及其受体Robo1参与肾输尿管芽的萌出、输尿管芽与生后肾组织的诱导、肾小体发育及泌尿小管发育和成熟过程。

Objective To study the expression changes of Slit2 and its receptor Robo1 in the kidneys of mice at different developmental stages.Methods The expressions of Slit2 and Robo1 were examined by immunohistochemical staining and Western blot.Results Slit2 and Robo1 began to be expressed in the nephrogenic regions of the mouse kidneys at the embryonic age of 12 days.Among them,Slit2 was positively expressed in the ureteric bud and the surrounding mesenchyme but not in other parts of the mice,while Robo1 was widely expressed in nephrogenic zone of the mice,mainly in the metanephrogenic tissue and the basal surface of the ureteric bud epithelium.In the developmental process of nephrons,Slit2 was strongly expressed in the comma body and S body stages,and weakly expressed in the stage III of renal corpuscle,the stage IV of renal corpuscle and the mature stage of renal corpuscle in the mice.Robo1 was strongly expressed in the stages of comma body and S body,the stage III of renal corpuscle and the stage IV of renal corpuscle,but weakly expressed in the mature stage of renal corpuscle in the mice.Both Slit2 and Robo1 were detected in proximal tubules,distal tubules and collecting ducts of the mice.The expression of Slit2 in the mice increased from the 14 day of embryo,reached the peak on the 16th day of embryonic age,and then gradually decreased.The expression of Robo1 protein in the mice gradually decreased from the 14th day of embryonic age.Conclusions The expressions of Slit2 and its receptor Robo1 in the renal corpuscles of developing stages and in the development and maturation of the uriniferous tubules in mice suggests that they may play an important role in the development of mouse renal corpuscles and uriniferous tubules.