肺组织修复中肺内外干细胞的作用、功能及问题

Stem cells inside and outside of the lung for lung repair: roles, functions and existing problems

背景:干细胞局部修复受损组织的能力为细胞治疗包括慢性肺病在内的大多数不可逆疾病提供了可能性。目的:阐述肺内外干细胞对肺组织的修复作用。方法:第一作者用计算机检索中国期刊全文数据库和Medline数据库,检索词分别为"干细胞,肺组织干细胞,气管-支气管上皮细胞,移植,细支气管-终末细支气管上皮细胞,肺泡上皮细胞,肺间质细胞"和"lung stem cells,tracheal and bronchial epithelial stem cells;transplantation;stem cells in the bronchial and terminal bronchial epithelium;stem cells at the bronchoalveolar junction;alveolar epithelial stem cells,interstitial lung stem cells",共计500余篇论文,语言分别设定为中文和英文,最终纳入47篇文献。结果与结论:肺内外干细胞是肺脏损伤、修复、重塑的基础,机体内肺干细胞多处于静息状态,当肺组织损伤或在体外培养的情况下,肺内外干细胞分裂、增殖。此外,在肺组织修复过程中甚至在生理条件下,是否由于其他因素的影响而发生细胞凋亡、迁移和分化,目前仍未知。因此,肺内外干细胞在慢性肺疾病中的作用仍有很多问题尚待解决。

BACKGROUND:Stem cells have the ability to repair local tissue damage,which opens the possibility of cell therapy for most irreversible diseases,including chronic lung disease.OBJECTIVE:To elucidate the effect of stem cells in the repair of lung tissue.METHODS:The first author used the computer to retrieve CNKI and Medline databases using the keywords of“lung stem cells,tracheal and bronchial epithelial stem cells;transplantation;stem cells in the bronchial and terminal bronchial epithelium;stem cells at the bronchoalveolar junction;alveolar epithelial stem cells,interstitial lung stem cells”in Chinese and English,respectively.Approximately 500 articles were initially retrieved,and finally 47 eligible articles were included.RESULTS AND CONCLUSION:Stem cells inside and outside of the lung are the basis of lung injury,repair and remodeling.The lung stem cells in the body are mostly in a resting state.When the lung tissue is damaged or cultured in vitro,the stem cells from the lung or other tissues divide and proliferate.In addition,it is still unknown whether apoptosis,migration and differentiation will be triggered by other factors during the repair of lung tissue or even under physiological conditions.Therefore,many problems regarding stem cells for chronic lung diseases remain to be solved.