基于部分还原氧化石墨烯纳米复合材料的肿瘤靶向光动力-光热联合治疗剂的制备

Preparation of a Targeted Tumor Nanocomposites for Combined Photodynamic-photothermal Therapy Based on Partially Reduced Graphene Oxide

以氧化石墨烯(GO)为原料,通过温和方法制备部分还原氧化石墨烯(pRGO);由pRGO与核酸适配体AS1411及吲哚菁绿(ICG)通过非共价作用构建了光热-光敏纳米复合物pRGO-AS1411-ICG(pRAI).利用傅里叶变换红外光谱(FTIR)、拉曼光谱(Raman)、X射线光电子能谱(XPS)、紫外-可见光谱、透射电子显微镜和EDS能谱对pRGO和pRAI复合物的结构和形貌进行了表征,并利用细胞实验考察了其肿瘤细胞的光疗-热疗效果.结果表明,pRAI以AS1411为靶向分子,可通过光热和光毒性双重作用高效杀伤肿瘤细胞.

Partially reduced grphene oxide(pRGO)is synthesized using a mild reduction method by incubating GO suspension under alkaline condition at room tempeature.Aptamer(AS1411)non-covalent binding with cardiogreen(ICG),a photosensitizer and photothermal effect molecule,to the surface of pRGO,was used to target cancer cells for photodynamic therapy(PDT)and photothermal therapy(PTT).Structure and morphology of the as-synthesized pRGO and pRGO-AS1411-ICG(pRAI)nanocomposites were confirmed by menas of FTIR,Raman,XPS,UV-Vis,transmission electron microscopy(TEM)and energy dispersive spectrometer(EDS).pRAI targets cancer cells with AS1411 to produce singlet oxygen for PDT and photothermal effect for PTT upon near-infrared(NIR)light irradiation.Since pRGO is decorated with several AS1411-ICG molecules,the pRGO-AS1411-ICG conjugate yields enhanced binding and therapeutic effect by the added ability to carry multiple photosensitizers.Consequently,pRAI offers a remarkably improved and synergistic therapeutic effect on cells.