循环肿瘤细胞与外周血检测非小细胞肺癌XRCC1多态性的相关研究

The Study Applying Circulating Tumor Cells and Peripheral Blood to Detect XRCC1 Gene Polymorphism

目的探讨循环肿瘤细胞(CTC)、外周血代替肿瘤组织进行X线修复交错互补基因(XRCC1)多态性检测的可行性。方法选取初治的非小细胞肺癌患者21例,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法分别检测患者的肿瘤组织、外周血、CTC的XRCC1Arg399位点的多态性,比较三者的一致性。结果 21例非小细胞肺癌患者的肿瘤组织、外周血、CTC中XRCC1的CC基因型分别为12,11,11例;CT基因型分别为7,9,8例;TT基因型分别为2,1,2例。肿瘤组织、外周血和CTC 3种方法检测XRCC1Arg399位点差别无统计学意义(χ2=0.950,P=0.978)。肿瘤组织和外周血的一致率为76.2%(16/21),和CTC一致率为95.3%(20/21)。CTC检测XRCC1 Arg399位点多态性一致性高于外周血检测。结论CTC及外周血中XRCC1Arg399多态性均可不同程度反映肿瘤组织中相关基因型的突变,但CTC准确性更高。

Objective To explore the feasibility of applying circulating tumor cells(CTCs)and peripheral blood instead of tumor tissues for detecting XRCC1 Arg399 polymorphism. Methods A total of 21 untreated patients with Non-small cell lung carcinoma(NSCLC)were recruited,and their tumor tissues,peripheral blood,and CTCs were analyzed for XRCC1 Arg399 polymorphism. Results from the 3 different sources(tumor tissues,peripheral blood,and CTCs)were compared against one another. Results The results revealed that the patient number of CC genotype of XRCC1 Arg399 polymorphism in tumor tissues,peripheral blood and CTCs was 12,11,11;CT genotype 7,9,8;and TT genotype 2,1,2. There was no significant difference among three approaches to detect XRCC1 Arg399 polymorphism.(χ2=0.950,P=0.978). An agreement between tumor tissues and peripheral blood was found(16/21,76.2%)and a significant correlation between tumor tissues and CTCs was observed(20/21,95.3%). The concordance between CTCs and tumor tissues was much higher than it was between tumor tissues and peripheral blood. Conclusion CTCs and peripheral blood may potentially be a substitute for tumor tissues to identity XRCC1 gene polymorphism,and CTCs appear to have higher accuracy.