芦苇-固定化微生物联合修复胶州湾湿地土壤中苯并［a］芘的研究

Remediation of Benzo[a]pyrene in Jiaozhou Bay Wetland Soil by Using Reed-Immobilized Microorganism Technology

从胶州湾湿地污染土壤中筛选出2株BaP降解菌—JB1和JB2,以改性煤渣、改性PVA为载体材料,将JB1、JB2混合菌通过包埋的方式制成固定化生物煤渣球并投加到种植了芦苇的BaP污染土壤中进行室外降解实验,42d后,BaP的降解率达到69.3%,降解效果好于生物煤渣球或芦苇单独作用时的降解效果。投加固定化微生物后,土壤中微生物群落的多样性明显减少,细菌的群落结构以PAHs降解菌为主,如Pseudomonas sp.,Mycobacteriumsp.,Achromobacter sp.;降解结束18d后,土壤中微生物群落的多样性增加,细菌的群落结构也得到一定程度的恢复。

Two strains(JB1 and JB2)were selected from BaP-contaminated sediments of Jiaozhou Bay wetland and immobilized in coal cinder beads using entrapping methods.The coal cinder gel-beads were input to the BaP-contaminated soil planted with reeds to implement outdoor degradation experiment.69.3%BaP was degraded by reed-coal cinder gel-beads combined system in 42 days,and the degradation was more effectively than the situation that BaP was degraded by reeds or coal cinder gel-beads alone.After the addition of coal cinder-gel beads,the microbial community diversity was decreased obviously and the community structure was dominated by the PAHs degraders,such as Pseudomonas sp.,Mycobacterium sp.,Achromobacter sp..However,the microbial community diversity was increased and the community structure was recovered to some extent in 18 days after the degradation.