中药消水方对Lewis肺癌小鼠恶性胸腔积液生成及水通道蛋白1表达的影响

Effect of Xiaoshui formula on the formation of malignant pleural effusion and the expression of aquaporin 1 in lung cancer mice

目的探讨中药消水方对肺癌小鼠恶性胸腔积液及水通道蛋白1(AQP1)表达的影响。方法将45只C57BL/6小鼠随机分为中药组、模型组、空白组。除空白组小鼠胸腔内注射PBS缓冲液外,其余两组小鼠均采用带荧光标记的Lewis肺癌细胞胸腔注射建立动物模型。连续给药10 d,并在第3、8、13天时进行活体成像观察,第14天处死取材,抽取胸腔积液测量体积,采用Real-Time PCR、Western blot法检测各组小鼠壁层胸膜AQP1 mRNA及蛋白表达水平。结果中药组小鼠胸腔荧光强度明显低于模型组(P <0. 05),胸腔积液体积也明显小于模型组(P <0. 01);模型组小鼠壁层胸膜AQP1 mRNA及蛋白表达相比于空白组均显著升高(P<0. 01);而中药组相比于模型组能显著降低壁层胸膜AQP1 mRNA及蛋白表达水平(P <0. 01)。结论肺癌恶性胸腔积液小鼠胸膜存在AQP1表达的上调,中药消水方可能通过下调壁层胸膜间皮细胞AQP1 mRNA及蛋白表达,从而促进恶性胸腔积液的转运,减少恶性胸腔积液的生成。

Objective To investigate the effect of Xiaoshui formula on the formation of malignant pleural effusion(MPE)and the expression of aquaporin 1(AQP1)in lung cancer mice.Methods 45 C57BL/6 mice were randomly divided into Chinese medicine group,model group and blank group.The blank group mice were injected intrathoracically with PBS buffer.The other two groups of mice were injected intrathoracically with fluorescent labeled Lewis lung carcinoma cells.During ten days of continuous administration,mice in vivo imaging were observed in third,eighth,thirteenth days.The pleural effusion was extracted and volume was measured when executed on the fourteenth day.Real-Time PCR and Western blot were used to detect the level of AQP1 mRNA and protein expression in the parietal pleura of mice.Results The fluorescence intensity of the thoracic cavity of the Chinese medicine group was significantly lower than that of the model group(P<0.05),and the volume of pleural effusion was also significantly smaller than that of the model group(P<0.01).The expression of AQP1 mRNA and protein in model mice parietal pleura were significantly increased compared to the blank group(both P<0.01).Compared with the model group,the Chinese medicine group can significantly reduce the parietal pleural AQP1 mRNA and protein expression levels(both P<0.01).Conclusion [WTBZ〗There is an up-regulation of AQP1 expression in the pleura of lung cancer MPE mice,and the traditional Chinese medicine Xiaoshui formula may promote MPE transport and reduce MPE production by down-regulating the expression of AQP1 mRNA and protein in pleural mesothelial cells.