加减薯蓣丸对轻、中度阿尔茨海默病患者血清特定microRNAs表达影响

Effect of Modified Yam Pills on Serum Specific MicroRNAs Expression in Patients with Mild and Moderate Alzheimer's Disease

目的探讨轻、中度阿尔茨海默病患者血清微小RNA(microRNA,miRNA)表达及加减薯蓣丸对其影响。方法检索Pubmed和中国知网数据库中研究AD患者外周血miRNAs文献,选择多篇(≥2篇)文献中一致性差异表达的miRNAs(至少1篇以血清为样本)作为目标miRNAs进行研究。56例轻、中度阿尔茨海默病患者给予加减薯蓣丸浓缩汤剂口服,每日2次,每次15mL,疗程12周。另收集同期认知功能正常的老年健康体检者56例为对照组。采用荧光定量PCR法检测AD患者治疗前后及对照组血清目标miRNAs水平。结果与对照组比较,AD组治疗前血清miR-107、miR-29a、miR-29b、miR-181c、miR-125b、miR-135a、miR-342-3p表达明显降低,miR-146a表达明显升高(P<0.01);与治疗前比较,AD组治疗后血清miR-107、miR-29a、miR-29b、miR-181c、miR-125b、miR-342-3p表达明显升高(P<0.05~0.01),miR-146a表达明显降低(P<0.01)。结论通过检测血清miR-107、miR-29a、miR-29b、miR-181c、miR-125b、miR-135a、miR-342-3p及miR-146a表达水平可能有助于AD患者早期诊断;加减薯蓣丸可能通过调节AD患者血清miR-107、miR-29a、miR-29b、miR-181c、miR-125b、miR-342-3p、miR-146a表达水平而发挥治疗AD的作用。

OBJECTIVE To explore the expression of serum microRNAs(miRNAs)in patients with mild and moderate Alzheimer's disease and the effect of modified Yam Pills on it.METHODS The literature of peripheral blood miRNAs in AD patients was retrieved in Pubmed and China National Knowledge Internet database.The miRNAs with consistent expression in multiple literatures were selected for the study.Totally 56 patients with mild and moderate Alzheimer's disease were treated with modified Yam Pills of 15 mL each time,twice a day for 12 weeks.Another 56 cases with normal cognitive function were collected as control group.The serum miRNAs levels of AD patients before and after treatment and control group were measured by fluorescence quantitative PCR.RESULTS Compared with the control group,the expression of serum miR 107,miR 29a,miR 29b,miR 181c,miR 125b,miR 135a and miR 342 3p were significantly decreased in the AD group,and the expression of miR 146a was significantly increased(P<0.01).Compared with those before treatment,the expression of serum miR 107,miR 29a,miR 29b,miR 181c,miR 125b and miR 342 3p were significantly increased after treatment in the AD group(P<0.05,P<0.01),and the expression of miR 146a was significantly decreased(P<0.01).CONCLUSION Detecting the expression of serum miR 107,miR 29a,miR 29b,miR 181c,miR 125b,miR 135a,miR 342 3p and miR 146a may be helpful to the early diagnosis of AD patients.Modified Yam Pills may play a role in the treatment of AD by regulating the levels of serum miR 107,miR 29a,miR 29b,miR 181c,miR 125b,miR 342 3p and miR 146a in AD patients.