氯硝柳胺磷酸酯通过抑制Wnt/β-catenin通路抑制肾脏组织纤维化

Phosphate niclosamide mitigates renal fibrosis through inhibiting Wnt/β-catenin signaling pathway

目的探讨氯硝柳胺磷酸酯(P-NICLO)抑制肾脏组织纤维化的作用及其可能机制。方法构建体内试验(UUO模型)及体外实验(TGF-β1体外刺激NRK-52E细胞导致细胞转分化)模型,予以一定浓度P-NICLO干预,然后通过病理染色,Western blot及Real-time PCR等生物信息学方法,检测P-NICLO对不同模型组肾脏组织纤维化的抑制作用及其对Wnt/β-catenin信号通路的影响。结果体内及体外实验均表明,P-NICLO可以明显抑制模型组肾脏组织纤维化,模型组Wnt/β-catenin信号通路也被明显抑制,差异有统计学意义(P<0.05)。结论 P-NICLO可能通过抑制Wnt/β-catenin信号通路而抑制肾脏组织纤维化。

Objective To explore the effect of p-niclosamide(P-NICLO)on renal fibrosis and its possible mechanism.Methods In vivo experiments(UUO model)and in vitro experiments(TGF-β1 stimulated NRK-52E cells in vitro),models were given P-NICLO intervention at a certain concentration to detect the inhibitory effect of P-NICLO on renal fibrosis in different model groups and its effect on Wnt/β-catenin signaling pathway by pathological staining,Western blot and Real-time PCR.Results Both in vivo and in vitro experiments showed that P-NICLO could significantly inhibit renal fibrosis and Wnt/β-catenin signaling pathway in model group.Conclusion P-NICLO may mitigate renal fibrosis through inhibiting the Wnt/β-catenin signaling pathway.