Pkhd1基因缺陷对雄性小鼠生育能力的影响

Effect of Pkhd1 gene deficiency on fertility in male mice

目的观察Pkhd1基因缺陷对雄性小鼠生育能力的影响。方法取同龄成年野生型和Pkhd1基因缺陷(Pkhd1^(-/-))小鼠各10只,取附睾尾精子,观察记录两组精子各项参数:精子浓度、活力、活率及形态;另外,取同龄成年野生型和Pkhd1^(-/-)小鼠各5只,将野生型雌鼠与之进行合笼试验,观察记录两组小鼠的各项生育指标:交配率(FM)、怀孕率(RP)及平均产仔率(ANP)。结果与野生型比较,Pkhd1基因缺陷小鼠精子浓度下降(P<0.01)、精子活力降低(P<0.01)且精子尾部形态异常;且与Pkhd1^(-/-)小鼠交配后的雌性小鼠RP显著下降(P<0.01)。结论 Pkhd1基因缺陷可降低雄性小鼠的生育力。此研究结果为男性常染色体隐性多囊肾患者在生育能力方面的潜在并发症提供了新的见解。

Objective To investigate the effect of Pkhd1 gene deficiency on fertility of male mice.Methods 10 adult wide-type mice and 10 Pkhd1-/-mice of same age were taken respectively and epididymal spermatozoa was separated to observe the sperm parameters of the two groups:sperm concentration,vitality and morphology;Furthermore,5 adult wide-type mice and 5 Pkhd1-/-mice of same age were taken respectively and the wild-type female mice were caged with two groups to observe the fertility indicators:frequency of mating(FM),rate of pregnancy(RP)and average number of pups(ANP).Results Compared with wild-type,sperm concentration in Pkhd1-deficient mice decreased(P<0.01),sperm motility decreased(P<0.01),and sperm tail morphology was abnormal;and RP in female mice mated with Pkhd1-/-mice declined significantly.Conclusion Pkhd1 deficiency is sufficient to reduce male fertility in mice.This study provides new insights into the potential complications of fertility in men with autosomal recessive polycystic kidney disease.