摘要
利用牛血清白蛋白(BSA)模板荧光铜纳米簇为荧光探针,发展了一种快速、非标记型的荧光生物传感新方法用于四环素的灵敏检测.结果表明,四环素的线性检测范围为5.0×10^(-8)～4.0×10^(-5)mol/L,检出限为5.0×10^(-9)mol/L(RSN=3).该方法已成功地应用于牛奶样品中四环素含量的测定,相对误差不大于3.3%,加标回收率在96.0%～102.8%之间.该研究为四环素的灵敏检测提供了一种快速、方便的新方法.
A rapid and label-free fluorescent sensing method has been established for sensitive detection of tetracycline(TC)by using bovine serum albumin(BSA)templated CuNCs as fluorescence probes.The strategy permitted sensitive detection of tetracycline in a linear range from 5.0×10-8 to 4.0×10-5 mol/L and with a detection limit of 5.0×10-9 mol/L.The sensor was successfully applied to determinate the tetracycline content in milk samples.It could provide a rapid and convenient fluorescent platform for tetracycline detection.
作者
王海波
董高丽
白宏宇
李阳
毛安丽
WANG Haibo;DONG Gaoli;BAI Hongyu;LI Yang;MAO Anli(Henan Province Key Laboratory of Utilization of Non-metallic Mineral in the South of Henan,Xinyang Normal University,Xinyang 464000,China;Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University,Xinyang 464000,China)
出处
《信阳师范学院学报（自然科学版）》
CAS
北大核心
2018年第4期632-635,共4页
Journal of Xinyang Normal University(Natural Science Edition)
基金
国家自然科学基金项目(U1704153
21305119)
河南省高等学校青年骨干教师培养计划(2017GGJS100)
