海洋来源的苏云金芽孢杆菌h3菌株和昆虫幼虫来源的Ly30对鳞翅目小菜蛾、双翅目家蝇和淡色库蚊、鞘翅目德国小蠊的毒性（英文）

The Toxicity of Bacillus thuringiensis Strains h3 from Oceans and Ly30 from Insect Larvae Against Diamondback Moth( Lepidoptera),Culex pipiens pallens and Musca domestica( Diptera),Blattella germanica( Coleoptera)

为发现和验证杀虫剂苏云金杆菌(Bt)菌株对目标种类的害虫的毒性,本文用两株菌种(h3和Ly30)对小菜蛾(鳞翅目)、淡色库蚊和家蝇(双翅目)和德国小蠊(鞘翅目)几种害虫进行了毒力检测.其中,菌株h3来源于连云港海水,具有高盐耐受的优点;菌株Ly30分离自连云港市云台山赤松林区自然死亡的膜翅目昆虫日本弓背蚁幼虫体内.杀虫活性实验显示,菌株h3和Ly30具有高效杀虫活力,对小菜蛾(鳞翅目)、淡色库蚊和家蝇(双翅目) 3 d～5 d致死率可达99%～100%,对德国小蠊(鞘翅目) 5 d致死率仅达30%. Bt菌株h3和Ly30对鳞翅目和双翅目害虫的强力杀虫活性及对鞘翅目害虫的毒力,不仅为其在农业、林业领域及公共卫生等方面的应用提供了理论支持.同时也拓展了Bt菌株毒杀害虫种类的研究.此外,具有高盐耐受优势的菌株h3不仅扩大了Bt生物杀虫剂的应用领域,也有利于增加适用对象,提高杀虫功效.

In order to find and identify more targets species of pests for toxic insecticidal Bacillus thuringiensis(Bt)strains,a toxicity survey was carried out of two strains h3 and Ly30 against diamondback moth(Lepidoptera),Culex pipiens pallens and Musca domestica(Diptera),Blattella germanica(Coleoptera),which were respectively isolated from Lianyungang oceans with excellent characteristic of high salt tolerance,and from insect Componotus iaponicu Mayr larvae,respectively.Bioassay showed that h3 and Ly30 have efficient insecticidal activity against diamondback moth(Lepidoptera),Culex pipiens pallens and Musca domestica(Diptera)by 99%-100%mortality at 3 d-5 d,while only have 30%mortality at 5 d against coleoptera pests.It provided strong evidence for the insecticidal role of autotrophic Bt strains h3 and Ly30 against pests populations of lepidoptera and diptera and coleoptera,and aslo evidence for the application in the fields of agriculture and forest and public health.Furthermore,high salt tolerance of h3 could provide great benefit not only in expanding the application fields of biological insecticides,but also increasing the applicable object,and enhancing the efficacy of Bt.