高效液相色谱法测定大米和小麦粉中的苯并(α)芘

Determination of Benzo(α)pyrene in rice and wheat flour by high performance liquid chromatography

以大米和小麦粉为样品,环己烷提取浓缩后用Phenomenex Luna C_(18)色谱柱分离,流动相为乙腈∶水=88∶12,流速为0.8 mL/min,荧光检测器(激发波长365 nm,发射波长410 nm)检测其中苯并(α)芘的含量。结果表明:苯并(α)芘在0.379~48.5μg/L范围内线性关系良好,相关系数r=0.999 9,在0.30~5.00μg/kg加标水平时,回收率为86.08%~98.17%,相对标准偏差为1.87%~4.22%(n=6);方法的检出限和定量限分别为0.1μg/kg和0.3μg/kg。该方法测定大米和小麦粉中的苯并(α)芘简单、快速、准确可靠。

With rice and wheat fl our as samples,Benzo(α)pyrene was extracted by cyclohexane,and separated by Phenomenex Luna C18 column with a mobile phase consisting of acetonitrile and water(88∶12)at 0.8mL/min after being concentrated.Benzo(α)pyrene was tested by fl uorescence detection with excitation wavelength 365nm and emission wavelength 410nm.Results indicated that the calibration curve was linear at the range of 0.379μg/L to 48.5μg/L with the correlation coeffi cient of 0.999 9.The recoveries ranged from 86.08%to 98.17%for Benzo(α)pyrene added between 0.30μg/kg to 5.00μg/kg with a relative standard deviation(RSD)of 1.87%to 4.22%(n=6).The limit of detection and limit of quantitation of the method were 0.1μg/kg and 0.3μg/kg,respectively.The method for determination of Benzo(α)pyrene in rice and wheat fl our was simple,fast,accurate and reliable.