椰子织蛾转录组分析

Transcriptome Analysis of Opisina arenosella

为获取入侵性检疫害虫椰子织蛾的基因信息,利用高通量测序技术对该害虫进行了转录组测序。结果表明:经拼接共获得了37 416条unigenes,平均长度为803 bp,N50为1 415 bp;通过BlastX比对,有19 154条unigenes (51.19%)成功在Nr中被注释。在Nr中成功注释的unigene,与黑脉金斑蝶同源性最高,达到68.45%;在GO注释中,共有14 131 (37.76%)条unigenes被成功注释到细胞组分、分子功能和生物过程3大类的54个分支;在COG数据库中,成功注释的8 606条unigenes被注释到26个分类;在KEGG中,共有6 171条(16.49%) unigenes被注释到5大类通路的260条途径上。基于构建的转录组数据库,鉴定得到3 248个微卫星。

1n order to obtain genetic data for Opisina arenosella,this study sequenced its transcriptome using high-throughput sequencing technology.1n total,37 416 unigenes were obtained.Their mean length was 803 bp with N50 of 1 415 bp.By BlastX search,a total of 19 154 unigenes were matched to the sequences in NCB1 non-redundant protein sequences(Nr)database.They had the highest similarity(68.45%)to those of Danaus plexippus.1n Gene Ontology(GO)database,14 131 unigenes(37.76%)were assigned to 54 branches of 3 categories including cellular component,biological processes and molecular function.1n Clusters of Orthologous Groups of proteins(COG)database,8 606 unigenes were assigned to 26 categories.Additionally,6 171 unigenes(16.49%)were mapped to Kyoto Encyclopedia of Genes and Genomes(KEGG)database,of which were divided into 260 known pathways of 5 metabolic pathways categories.1n total,3 248 microsatellites were identified from the transcriptome database.