缓冲液种类对土壤酸性磷酸酶活性的影响

Effects of buffer on soil acid phosphatase activity

土壤酸性磷酸酶影响土壤有机磷矿化。选择不同的缓冲液种类和不同p H值测定土壤酸性磷酸酶活性,可影响数据的可比性。为明确缓冲液及p H值对不同土壤磷酸酶活性的影响程度,选取p H值4～8的土壤样本,测定了在醋酸盐缓冲液、柠檬酸盐缓冲液和MUB缓冲液3种不同培养条件下酸性磷酸酶活性变化。结果表明,采用MUB缓冲液条件可获得较稳定的土样区分序列,且与缓冲液的p H值的变化无关。在醋酸盐缓冲液和柠檬酸盐缓冲液条件下,不同p H值范围的不同土样之间土壤酸性磷酸酶活性变异系数变化剧烈,而采用MUB缓冲液获得的变异系数较其他缓冲液大,且趋势平稳。综合考虑,进行土壤酸性磷酸酶活性测定,可优先选用MUB缓冲溶液。

The soil acid phosphatase is closely related to soil organic phosphorus mineralization.The choice of buffers and pH may result in different results of enzyme activity determination.In order to figure out the change of regulation,the acid phosphatase activity of different soil under acetate buffer,citrate buffer and MUB buffer were measured.The results showed that a stable soil sample sequence can be obtained by using the MUB buffer solution,and it is not related to the change of pH in the buffer solution.Under the conditions of acetate buffer and the citrate buffer,the variation coefficient of soil acid phosphatase activity was drastic changes in range of different pH among different soils.But the variation coefficient of soil acid phosphatase activity under the condition of MUB buffer was large and trended stationary.In a short,MUB buffer solution was proposed for determining the soil acid phosphatase activity.