二冬汤和有氧运动对2型糖尿病大鼠糖代谢和脂代谢的影响

Effect of ErDong decoction and aerobic exercise on glucose metabolism and lipid metabolism in type 2 diabetes rats

目的探讨二冬汤和有氧运动对2型糖尿病(T2DM)大鼠糖代谢和脂代谢作用的影响。方法采用小剂量腹腔内注射链脲佐菌素(STZ,30mg/kg)加高糖高热量饲料喂养的方法诱发并建立2型糖尿病大鼠模型。将2型糖尿病模型组动物分为模型组(DM)、有氧运动组(CE)、二冬汤组(ED)、有氧运动+二冬汤组(CE+ED),另设正常对照组(NC)。有氧运动和药物干预6周后,观察并检测各组动物空腹血糖(FBG)、糖化血红蛋白(HbA1c)、血清胰岛素(Ins)、总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、超氧化物歧化酶(SOD)、丙二醛(MDA)表达水平。结果与NC组比较,DM组在持续喂养4周高糖高热量饲料,体质量增加明显,差异有统计学意义(P<0.05),大鼠FBG水平明显升高,差异有统计学意义(P<0.01);与DM组相比较,CE+ED组大鼠TC、TG、LDL-C、Ins、GSH水平和SOD活性显著升高(P<0.05);大鼠FBG、HbA1c、HDL-C和MDA水平显著降低(P<0.01)。与CE、ED组分别比较,CE+ED组对大鼠血糖和血脂水平变化差异有统计学意义(P<0.01)。结论有氧运动与二冬汤结合可明显降低T2DM模型大鼠血糖和血脂水平,促进血胰岛素的分泌,增强体内抗氧化酶活性,从而纠正和改善T2DM模型大鼠糖代谢和脂代谢紊乱。

Objective To explore the effect of ErDong decoction and aerobic exercise on glucose metabolism and lipid metabolism in type 2 diabetes mellitus(T2DM)rats.Methods The rats of T2DM models were induced by low-dose intraperitoneal injection of streptozotocin(STZ,30 mg/kg)plus high-sugar and high-calorie diet feeding.The T2DM model group were randomly divided into Model group(DM),Aerobic exercise group(CE),ErDong decoction group(ED)and Aerobic exercise+ErDong decoction(CE+ED).Meanwhile,normal control group(NC)was set up.Rats in ED group and CE+ED group were given 2 g/kg Erdong decoction,and the rats in CE group and CE+ED group were subjected to a weight-free swimming exercise for 60 min,5 times a week.After 6 weeks of aerobic exercise and drug intervention,fasting blood glucose(FBG),glycosylated hemoglobin(HbAlc),serum insulin(Ins),total cholesterol(TC),triglyceride(TG),high density lipoprotein protein(HDL-C),low density liprotein protein(LDL-C),superoxide dismutase(SOD),malondialdehyde(MDA)were observed and tested.Results Compared with NC group,the DM group maintained a high-sugar and high-calorie diet for 4 weeks,the body weight gained obversly(P<0.05).The level of FPG in rats was significantly higher(P<0.01).Compared with DM group,the levels of TC,TG,LDL-C,Ins,GSH and SOD in CE+ED group were significantly increased(P<0.01);FBG,HbAlc and MDA levels were significantly reduced(P<0.01).Compared with CE and ED respectively,and CE+ED group had a more significant regulation effect on blood glucose and blood lipid(P<0.01).Conclusion The combination of aerobic exercise and Erdong decoction can significantly reduce the blood glucose and blood lipid levels of T2DM model rats,promote the secretion of blood insulin and enhance the activity of antioxidant enzymes,thus correcting and improving the glucose metabolism and lipid metabolism disorder in T2DM model rats.