摘要
对橙黄玉凤花Habenaria rhodocheila种子进行无菌萌发培养以获得大量原球茎,并对原球茎组培,建立其快繁体系。结果表明,橙黄玉凤花种子以0.1%升汞消毒15 min为最佳处理。种子萌发培养基中,添加0.2%活性碳(AC)有利于种子萌发。种子萌发的最佳培养基为1/2MS+NAA 0.5 mg·L^(-1)+AC 0.2%,原球茎增殖分化最佳培养基为1/2MS+NAA 0.2 mg·L^(-1)+ZT 3.0 mg·L^(-1)+AC 0.2%;生根最佳培养基为1/2MS+NAA 0.1 mg·L^(-1)+6-BA 2.0 mg·L^(-1)+AC 0.2%。
Effects of different disinfection treatments,additives,plant growth regulators on tissue culture and rapid propagation system of Habenaria rhodocheila with seeds as explants were studied.Sterilizing the seeds with 0.1%mercuric chloride for 15 min was the best disinfection treatment.Seeds germinated only on the medium adding active carbon(AC).0.2%active carbon in culture medium was fit for germination.The optimal medium for seed germination was 1/2MS+NAA 0.5 mg·L-1+AC 0.2%,the optimal medium for multiple buds formation was 1/2MS+NAA 0.2 mg·L-1+ZT 3.0 mg·L-1+AC 0.2%,the optimal rooting medium was 1/2MS+NAA 0.1 mg·L-1+6-BA 2.0 mg·L-1+AC 0.2%.
作者
郑洲翔
刘德浩
陈智涛
廖建良
ZHENG Zhou-xiang;LIU De-hao;CHEN Zhi-tao;LIAO Jian-liang(School of Life Science,Huizhou University,Huizhou 516007,Guangdong China;Huizhou Institute of Forestry,Huizhou 516001,Guangdong China)
出处
《亚热带植物科学》
2018年第3期273-276,共4页
Subtropical Plant Science
基金
广东省科技厅科技基础条件研究项目(2015A030302007)
广东省科技计划项目(2017A020213031)
惠州市科技计划项目(2016x0427042)
广东省植物学资源共享课程(粤教函2017(214)号)
关键词
橙黄玉凤花
组织培养
快繁
Habenaria rhodocheila
tissue culture
rapid propagation