大花蕙兰组织培养技术研究

Tissue Culture Technology of Cymbidium hybridum

以大花蕙兰Cymbidium hybridum侧芽为外植体,以N6、B5、CC、MS为基本培养基,设计无机大量元素、无机微量元素、有机物三因素四水平正交试验,探究大花蕙兰丛生芽增殖的最佳基本培养基、生长调节剂浓度配比以及诱导生根的最佳生长调节剂浓度。结果显示,大花蕙兰丛生芽增殖的最佳培养基组合为B5无机大量元素+CC无机微量元素+MS有机物+蔗糖30 g·L^(-1)+琼脂7 g·L^(-1)+活性炭0.5 g·L^(-1)(p H 5.8~6.0),诱导丛生芽增殖的适宜生长调节剂浓度配比为6-BA 4.0 mg·L^(-1)+NAA 0.2 mg·L^(-1),诱导生根的最佳生长调节剂浓度为NAA 1.0 mg·L^(-1)。

To set up a new tissue culture system with the buds of Cymbidium hybridum as tissue explants,N6,B5,CC and MS as basic medium,orthogonal experiment design was carried out with macroelement,microelement and organic matter as three different influencing factors.Results showed that the best medium combination for developing C.hybridum was B5 macroelement+CC microelement+MS organic+sucrose 30 g·L-1+agar 7 g·L-1+activated carbon 0.5 g·L-1(pH 5.8-6.0),the best medium hormone concentration combination for multiple bud proliferation was 6-BA 4.0 mg·L-1+NAA 0.2 mg·L-1,the best hormone concentration for induce in vitro shoot rooting was NAA 1.0 mg·L-1.